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Invitrogen
Hex Polyclonal Antibody
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FIGURE: 1 / 2
Hex Antibody (720258) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized HepG2 cells for detection of endogenous HHEX using anti-HHEX Rabbit Polyclonal Antibody (Product # 720258, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of HHEX protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) rep... View More
Product Details
720258
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Protein corresponding to Human HHEX (aa 198-212, 216-230)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Dog, Cat and Bovine
Target Information
HHEX is a member of the homeobox family of transcription factors, many of which are involved in developmental processes. Expression in specific hematopoietic lineages suggests that this protein may play a role in hematopoietic differentiation. Establishes anterior identity at two levels 1) acts early to enhance canonical WNT-signaling by repressing expression of TLE4 and 2) acts later to inhibit NODAL-signaling by directly targeting NODAL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hematopoietically-expressed homeobox protein HHEX; homeobox; Homeobox protein HEX; Homeobox protein PRH; homeobox, hematopoietically expressed; huprobox; Proline-rich homeodomain protein; proline-rich homeodomain-containing transcription factor; unnamed protein product
Gene Aliases: HEX; HHEX; HMPH; HOX11L-PEN; PRH; PRHX
UniProt ID: (Human) Q03014
Entrez Gene ID: (Human) 3087
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein binding
eukaryotic initiation factor 4E binding
DNA binding, bending
TBP-class protein binding
protein homodimerization activity
sequence-specific DNA binding
translation regulator activity
DNA-binding transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
anterior/posterior pattern specification
negative regulation of transcription by transcription factor localization
regulation of mRNA export from nucleus
negative regulation of transcription by competitive promoter binding
Wnt signaling pathway
negative regulation of angiogenesis
cell differentiation
positive regulation of Wnt signaling pathway
B cell differentiation
negative regulation of vascular endothelial growth factor receptor signaling pathway
protein localization to nucleus
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of leukocyte proliferation
DNA conformation change
positive regulation of canonical Wnt signaling pathway
negative regulation of cytoplasmic translational initiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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