Rockland

Hif3 alpha Polyclonal Antibody

Name: Hif3 alpha Polyclonal Antibody
Brand: Rockland
SKU: 600-401-435

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Hif3 alpha Antibody in Western Blot (WB)

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Hif3 alpha Antibody (600-401-435) in WB

Western blot using Rocklands Affinity Purified anti-Hif3A antibody shows detection of a band ~72 kDa corresponding to mouse Hif3A (arrowhead). Approximately 10 µg of a CoCl₂ treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 µM CoCl₂ for 6 h at 37° C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4° C with the primary antibody diluted to 1:1,6... View More

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Immunogen Image Hif3 alpha Polyclonal Antibody

产品信息

600-401-435

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500- 1:2,000

酶联免疫吸附实验 (ELISA)

1:14,000-1:80,000

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 a (Hypoxia Inducible Factor).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.2 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity-purified antibody is directed against mouse Hif3alpha protein. A BLAST analysis was used to suggest cross reactivity with Hif3a protein from mouse (100% homology) and rat (91% homology). Reactivity against human Hif3a is not expected as only 75% homology for the immunizing sequence is noted. No reactivity is expected against other forms of Hif proteins. Reactivity against homologues from other sources is not known.

靶标信息

Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1 alpha, HIF-1 beta (also designated Arnt 1), EPAS-1 (also designated HIF-2 alpha) and HIF-3 alpha, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1 alpha is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Basic-helix-loop-helix-PAS protein MOP7; HIF-3-alpha; HIF3-alpha; HIF3-alpha-1; hypoxia inducible factor three alpha; Hypoxia-inducible factor 3-alpha; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; Neonatal and embryonic PAS protein

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基因别名: bHLHe17; Hif3a; Ipas; MOP7; NEPAS

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UniProt ID: (Mouse) Q0VBL6

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Entrez Gene ID: (Mouse) 53417

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功能

RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity transcription corepressor activity signal transducer activity protein binding protein dimerization activity basic helix-loop-helix transcription factor

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