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Human VEGF-165 Protein (PHC9391) in Bioactivity
Immunofluorescence analysis of macrophage migration inhibitory factor (MIF) was performed using U-87 MG cells treated with 100 ng/mL human VEGF-165 recombinant protein (Product # PHC9391 ) for 48 hours. The cells were labeled with MIF polyclonal antibody (Product # PA5-81446) (Panel a & c; Green). The composite (b & d) represents merged image with DAPI (blue) and F-actin (red) staining. Cells treated with V... View More
产品信息
PHC9391
应用
建议稀释比
已发表文章
产品规格
种属
Human
已发表种属
Human,
Mouse
Expression System
HEK293 cells
氨基酸序列
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
分子量
40 kDa
分类
Recombinant
类型
Protein
纯度
≥95% by SDS-PAGE and HPLC
内毒素浓度
<0.1 ng/µg
活性
ED50 < 6 ng/mL; determined by the dose-dependent proliferation of HUVECs.
偶联物
形式
Lyophilized
纯化类型
purified
内含物
no preservative
保存条件
4°C
运输条件
Wet ice
产品详细信息
Carrier-Free
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water or appropriate buffered solution containing 0.1% BSA to regain full activity. Make any further dilutions of the reconstituted protein in buffered solution containing a carrier protein such as PBS with 0.1% BSA.
Storage: Store the lyophilized protein at 2-8°C or -20°C for long term storage, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
靶标信息
The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: H-VEGF-165; L-VEGF; M-VEGF-165; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; VEGF165; VPF
基因别名: MVCD1; VEGF; VEGFA; VPF
UniProt ID: (Human) P15692
Entrez Gene ID: (Human) 7422
