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Agrisera
HydA/Iron-hydrogenase HydA1/HydA2 Polyclonal Antibody
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HydA/Iron-hydrogenase HydA1/HydA2 Antibody (AS09-514) in WB
50 ng of purified protein (Hyd A 1 and Hyd A 2) were separated on 10% SDS-PAGE and blotted 25 min to PVDF membrane. Filters were blocked 1 h with 3% low-fat milk powder in PBS-T (0.1% TWEEN 20) and probed with anti-Hyd A 1/2 (AS09 514,1:5,000 over night at 4°C) and secondary anti-rabbit (1:10,000 1 h) antibody (HRP conjugated, manufacture Pierce) in PBS-T containing 3% low fat milk powder. Antibody incubati... View More
产品信息
AS09-514
产品规格
种属反应
Algae
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant, full length Chlamydomonas reinhardtii HydA-2.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Chlamydomonas reinhardtii
靶标信息
HydA encodes the catalytic subunit of an [FeFe]-hydrogenase that is typically a soluble enzyme found in the cytoplasm of anaerobic microorganisms, where it interfaces with cellular redox carriers to support fermentative and other anaerobic metabolic pathways. The HydA protein scaffold binds the defining H-cluster active site, which consists of a canonical [4Fe-4S] cubane linked through a cysteine thiolate to a distinctive 2Fe subcluster bearing non-protein ligands (including CO, CN, and a bridging dithiolate), forming the organometallic center responsible for catalysis; structural studies of apo-forms lacking the 2Fe module show a preformed [4Fe-4S] subcluster and an open pocket that accommodates stepwise insertion of the remaining 2Fe unit during maturation. Functionally, HydA catalyzes the reversible reduction of protons to molecular hydrogen (H2) and the oxidation of H2 back to protons and electrons, thereby helping balance intracellular redox and enabling hydrogen production or consumption depending on metabolic context; full activity depends on proper H-cluster assembly, in which general Fe-S biogenesis machinery provides the cubane and dedicated maturation factors assemble and deliver the 2Fe subcluster.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HYDA1; HYDA2
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