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Invitrogen
Hygromycin B Recombinant Mouse Monoclonal Antibody (1G12)
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Hygromycin B Antibody (MA566461) in SDS-PAGE
SDS-PAGE characterization of Hygromycin B under reducing conditions. Lane M: Protein marker (kDa); Lane HMB: Purified Hygromycin B Recombinant Monoclonal (Product # MA566461). Hygromycin B showing heavy chain (~50 kDa) and light chain (~25 kDa). Gels were stained with Coomassie Brilliant Blue R-430.
产品信息
MA566461
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG
Expression System
CHO cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
1G12
抗原
Conjugate of HMB hapten and KLH.
偶联物
形式
Liquid
浓度
8.6 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
Hygromycin B is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus. It functions by inhibiting protein synthesis, affecting both prokaryotic and eukaryotic cells. Hygromycin B binds to 16S rRNA, specifically near the A site at the top of helix 44, causing miscoding during translation, although less so than other aminoglycosides. It is particularly effective in halting yeast cell growth by preventing protein synthesis in cytoplasmic ribosomes. This antibiotic is used in molecular biology as a selection marker for genetically modified organisms due to its ability to kill bacteria, fungi, and higher eukaryotic cells. Structurally, hygromycin B is characterized by its interaction with the ribosomal RNA, differing from other antibiotics like paromomycin and streptomycin.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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