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Invitrogen
ID3 Monoclonal Antibody (2B11)
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ID3 Antibody (MA1-23242) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of ID3 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ID3 Monoclonal Antibody (2B11) (Product # MA1-23242) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
产品信息
MA1-23242
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2B11
抗原
The full-length (amino acids 1-119) mouse Id3 gene expressed in E. coli.
偶联物
形式
Liquid
浓度
2.45 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: Id3-transfected 293T, Id3 transfected Sol8 muscle cells and recombinant fusion protein.
靶标信息
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: bHLHb25; Class B basic helix-loop-helix protein 25; DNA-binding protein inhibitor ID-3; HEIR 1; Helix-loop-helix protein HEIR-1; HLH642; ID 3; ID-like protein inhibitor HLH 1R21; ID-like protein inhibitor HLH 462; Inhibitor of differentiation 3; Inhibitor of DNA binding 3; inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
基因别名: 1R21; BHLHB25; HEIR-1; HEIR1; Hlh462; Id-3; ID3; Idb3
UniProt ID: (Human) Q02535, (Mouse) P41133
Entrez Gene ID: (Human) 3399, (Mouse) 15903
negative regulation of transcription from RNA polymerase II promoter
metanephros development
regulation of DNA replication
transcription, DNA-templated
multicellular organism development
central nervous system development
heart development
muscle organ development
circadian rhythm
response to wounding
neuron differentiation
epithelial cell differentiation
notochord development
odontogenesis
positive regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of myoblast differentiation
negative regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
regulation of cell cycle
cellular response to leptomycin B
regulation of transcription, DNA-templated
rhythmic process
