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Invitrogen
IDO Recombinant Rabbit Monoclonal Antibody (PD00-62)
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IDO Antibody (MA5-50819) in ICC/IF
Immunocytochemistry analysis of IDO with HeLa cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp), incubated with recombinant monoclonal IDO (Product # MA5-50819) at a dilution of 1:100 (in 1% BSA in PBST overnight at 4°C), followed by Goat Anti-Rabbit IgG H&L 488 at 1:1,000 dilution (PBS used in control). Nuclear DNA was labelled in blue with DAPI, Beta tubulin (red) at 1/100 dilution (overn... View More
产品信息
MA5-50819
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PD00-62
抗原
Synthetic peptide derived from the C-terminus of human IDO-1 protein aa 350-403/403.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive control: Human endometrial carcinoma tissue, human lymph nodes tissue, human placenta tissue.
Predicted band size: 45 kDa
Subcellular Location: Cytoplasm.
靶标信息
IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EC 1.13.11.52; IDO-1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; Indoleamine 2,3-dioxygenase 1; Indoleamine-2; Indoleamine-pyrrole 2,3-dioxygenase
基因别名: IDO; IDO-1; IDO1; INDO
UniProt ID: (Human) P14902
Entrez Gene ID: (Human) 3620
immune system process
cytokine production involved in inflammatory response
positive regulation of T cell tolerance induction
positive regulation of chronic inflammatory response
positive regulation of type 2 immune response
tryptophan catabolic process
female pregnancy
tryptophan catabolic process to kynurenine
response to lipopolysaccharide
negative regulation of interleukin-10 production
positive regulation of interleukin-12 production
multicellular organismal response to stress
kynurenic acid biosynthetic process
swimming behavior
negative regulation of T cell proliferation
regulation of activated T cell proliferation
oxidation-reduction process
negative regulation of T cell apoptotic process
positive regulation of T cell apoptotic process
