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Invitrogen
IDO Recombinant Mouse Monoclonal Antibody (V1NC3IDO), Alexa Fluor™ Plus 488
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IDO Antibody (740117MP488) in WB
Western blot (Fig. a) was performed using IDO Recombinant Mouse Monoclonal Antibody (V1NC3IDO), Alexa Fluor™ Plus 488 (Product # 740117MP488) and an ~43 kDa band corresponding to IDO was observed upon Interferon gamma treatment. Whole cell extracts (60 µg lysate) of A549 (Lane 1), and A549 treated with Human IFN gamma (50 ng/mL) for 24 hours (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot™ 2 Dry ... View More
产品信息
740117MP488
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
V1NC3IDO
偶联物
激发/发射光谱
493/518 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EC 1.13.11.52; IDO-1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; indoleamine 2,3-dioxygenase (IDO) (EC 1.13.11.17); Indoleamine 2,3-dioxygenase 1; Indoleamine-2; Indoleamine-pyrrole 2,3-dioxygenase; unnamed protein product
基因别名: IDO; IDO-1; IDO1; INDO
UniProt ID: (Human) P14902
Entrez Gene ID: (Human) 3620
immune system process
positive regulation of T cell tolerance induction
positive regulation of chronic inflammatory response
positive regulation of type 2 immune response
tryptophan catabolic process
inflammatory response
female pregnancy
tryptophan catabolic process to kynurenine
quinolinate biosynthetic process
response to lipopolysaccharide
negative regulation of interleukin-10 production
positive regulation of interleukin-12 production
multicellular organismal response to stress
kynurenic acid biosynthetic process
'de novo' NAD biosynthetic process from tryptophan
swimming behavior
T cell proliferation
negative regulation of T cell proliferation
positive regulation of apoptotic process
regulation of activated T cell proliferation
negative regulation of T cell apoptotic process
positive regulation of T cell apoptotic process
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