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Invitrogen
IFI16 Monoclonal Antibody (2I3D7)
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IFI16 Antibody (MA5-49174) in ICC/IF
Immunocytochemistry analysis of IFI16 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in IFI16 Monoclonal antibody (Product # MA5-49174) using a dilution of 5 μg/mL. DyLight®550 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
产品信息
MA5-49174
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
2I3D7
抗原
E.coli-derived human IFI16 recombinant protein (Position: E183-K743).
偶联物
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Daudi whole cell, human Raji whole cell, human MOLT-4 whole cell, human U-87 MG whole cell. ICC/IF: A431 cell. Flow: U251 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
IFI16 (gamma-inteferon-inducible protein 16) binds double-stranded DNA. It binds preferentially to supercoiled DNA and cruciform DNA structures. It seems to be involved in transcriptional regulation and as a transcriptional repressor. IFI16 may have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. It controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. IFI16 is also involved in innate immune response by recognizing viral dsDNA in the cytosol and nucleus. After binding to viral DNA in the cytoplasm, IFI16 recruits TMEM173/STING and mediates the induction of IFN-beta. It is necessary to activate the IFR3 signaling cascade suring human herpes simplex virus 1 infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibiton of viral immediate-early gene expression and replication. IFI16 is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Gamma-interferon-inducible protein 16; IFI 16C; Ifi-16; IFI16; interferon-gamma induced protein IFI 16; Interferon-inducible myeloid differentiation transcriptional activator; unnamed protein product
基因别名: IFI16; IFNGIP1; PYHIN2
UniProt ID: (Human) Q16666
Entrez Gene ID: (Human) 3428
negative regulation of transcription from RNA polymerase II promoter
positive regulation of cytokine production
activation of innate immune response
immune system process
autophagy
apoptotic process
inflammatory response
regulation of autophagy
myeloid cell differentiation
monocyte differentiation
positive regulation of interleukin-1 beta production
cellular response to interferon-beta
cellular response to glucose starvation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
negative regulation of DNA binding
negative regulation of viral genome replication
innate immune response
negative regulation of gene expression, epigenetic
negative regulation of innate immune response
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
defense response to virus
cellular response to ionizing radiation
intrinsic apoptotic signaling pathway by p53 class mediator
negative regulation of AIM2 inflammasome complex assembly
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