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Invitrogen
IFNAR1 Monoclonal Antibody (MAR1-5A3), Functional Grade, eBioscience™
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IFNAR1 Antibody (16-5945-85) in Neu
Frontiers in immunology 2023 -
Dexamethasone inhibits expression of IFNbeta in LPS-activated mouse macrophages via an unknown mechanism. (A) A RAW264.7 cell line stably transfected with an ISGF3-dependent SEAP (secreted alkaline phosphatase) reporter was treated with combinations of LPS (10 ng/mL), IFNbeta (10 ng/mL), Ruxolitinib (Rux) and Dex as indicated for 24 h and reporter activity was assayed. The graph shows mean results from 3-6 ... View More
产品信息
16-5945-85
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Bacteria,
C. elegans,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
MAR1-5A3
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The MAR1-5A3 monoclonal antibody reacts with mouse IFN alpha/beta receptor subunit 1 (IFNAR-1). The type I IFN receptor subunit 1 is co-expressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs, IFN alpha and IFN beta. Type I IFNs are a group of structurally and functionally related proteins that demonstrate antiviral, antiparasitic, and antiproliferative activities.
Applications Reported:The MAR1-5A3 antibody has been reported for use in neutralization of IFNAR1 bioactivity.
Applications Tested: The Functional Grade Purified MAR1-5A3 antibody has been tested by LAL assay to verify low endotoxin levels, by bioassay for neutralization of mouse IFNAR-1 bioactivity. The MAR1-5A3 antibody at 0.04 µg/mL has been found to inhibit by 50% the biological effects of 0.5 ng/mL mouse IFN-a2 (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in BestProtocols® section.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation:Less than 10%, as determined by HPLC.
靶标信息
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron,2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-& Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: H3K1; IFN R1; IFN-alpha/beta receptor 1; IFN-alpha/betaR; IFN-alpha/betaR1; IFN-alpha/betaRalpha; IFN-R-1; IFNalpha/beta R1; IFNalpha/beta-Ralpha; IFNalpha/betaR1; IFNR1; INF-a receptor; Interferon alpha/beta receptor 1; interferon-alpha/beta receptor 1; Type I interferon receptor 1
基因别名: CD118; Ifar; Ifnar; Ifnar1; Ifrc; Infar
UniProt ID: (Mouse) P33896
Entrez Gene ID: (Mouse) 15975
