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Invitrogen
IKK beta Monoclonal Antibody (10A9B6)
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IKK beta Antibody (MA5-16162) in WB
Western blot analysis of IKK beta in HeLa lysate (1.0 mg/mL). Samples were incubated with IKK beta monoclonal antibody (Product # MA5-16162). Analysis using Azide Free version. Lane view shows a specific band for IKK beta. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
产品信息
MA5-16162
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
10A9B6
抗原
Full-length human IKKb/IKK2 recombinant protein.
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Suggested positive control: Daudi, HeLa, Jurkat, or NIH-3T3.
靶标信息
Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kaapa-B signaling pathway which is activated by mulitple stimuli such as inflammatory cytokines, bacterial, or viral products, DNA damages, or other cellular stresses. IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: FLJ40509; I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK-2; IKK-B; IKK2; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta; Serine/threonine protein kinase IKBKB
基因别名: AI132552; IKBKB; IKK-2; IKK-beta; IKK2; IKK[b]; IKKB; IKKbeta; IMD15; NFKBIKB
UniProt ID: (Human) O14920, (Mouse) O88351
Entrez Gene ID: (Human) 3551, (Mouse) 16150
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
IkappaB kinase activity
protein kinase binding
protein homodimerization activity
protein heterodimerization activity
scaffold protein binding
nucleotide binding
receptor signaling protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein modifying enzyme
stimulatory C-type lectin receptor signaling pathway
skeletal muscle contraction
protein phosphorylation
inflammatory response
I-kappaB kinase/NF-kappaB signaling
I-kappaB phosphorylation
positive regulation of cell proliferation
response to virus
response to toxic substance
regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of neuron projection development
cortical actin cytoskeleton organization
neuron projection development
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
negative regulation of myosin-light-chain-phosphatase activity
TRIF-dependent toll-like receptor signaling pathway
Fc-epsilon receptor signaling pathway
regulation of phosphorylation
response to drug
serine phosphorylation of STAT protein
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
stress-activated MAPK cascade
nucleotide-binding oligomerization domain containing signaling pathway
interleukin-1-mediated signaling pathway
response to fatty acid
cellular response to tumor necrosis factor
establishment of protein localization to plasma membrane
positive regulation of neuron death
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
B cell homeostasis
positive regulation of sodium ion transport
phosphorylation
signal transduction by protein phosphorylation
positive regulation of cation channel activity
