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IL-1 beta Antibody (710270) in ICC/IF
Immunofluorescence analysis of Interleukin-1 beta was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IL-1 beta Recombinant Rabbit Superclonal™ Antibody (1HCLC) (Product # 710270) at 5µg/mL in 0.1% BSA... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
710270
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
1HCLC
抗原
Recombinant protein corresponding to amino acids 118-269 of mouse IL-1b
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta
基因别名: Il-1b; IL-1beta; IL1B; IL1F2
UniProt ID: (Mouse) P10749
Entrez Gene ID: (Mouse) 16176
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
activation of MAPK activity
fever generation
positive regulation of protein phosphorylation
inflammatory response
immune response
positive regulation of cytosolic calcium ion concentration
aging
memory
negative regulation of cell proliferation
response to carbohydrate
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
negative regulation of gene expression
negative regulation of glucose transport
positive regulation of cell death
negative regulation of glutamate secretion
cytokine-mediated signaling pathway
hyaluronan biosynthetic process
neutrophil chemotaxis
sequestering of triglyceride
positive regulation of fever generation
lipopolysaccharide-mediated signaling pathway
positive regulation of prostaglandin secretion
response to lipopolysaccharide
interleukin-1 beta production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of stress-activated MAPK cascade
positive regulation of immature T cell proliferation in thymus
response to ATP
positive regulation of heterotypic cell-cell adhesion
social behavior
ectopic germ cell programmed cell death
positive regulation of myosin light chain kinase activity
cellular response to drug
positive regulation of T cell proliferation
positive regulation of NF-kappaB import into nucleus
positive regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of MAP kinase activity
protein kinase B signaling
positive regulation of JUN kinase activity
positive regulation of chemokine biosynthetic process
positive regulation of interleukin-2 biosynthetic process
positive regulation of interleukin-6 biosynthetic process
positive regulation of nitric oxide biosynthetic process
negative regulation of neuron differentiation
positive regulation of glial cell differentiation
positive regulation of angiogenesis
negative regulation of lipid metabolic process
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
negative regulation of insulin receptor signaling pathway
positive regulation of astrocyte differentiation
positive regulation of phagocytosis
negative regulation of neurogenesis
regulation of insulin secretion
leukocyte migration
negative regulation of lipid catabolic process
positive regulation of lipid catabolic process
positive regulation of membrane protein ectodomain proteolysis
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
positive regulation of cell division
positive regulation of calcidiol 1-monooxygenase activity
negative regulation of adiponectin secretion
positive regulation of ERK1 and ERK2 cascade
monocyte aggregation
cellular response to organic substance
cellular response to organic cyclic compound
positive regulation of monocyte chemotactic protein-1 production
positive regulation of neutrophil chemotaxis
extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of branching morphogenesis of a nerve
negative regulation of neural precursor cell proliferation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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