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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • IL-1 beta Antibodies

          Invitrogen

          IL-1 beta Monoclonal Antibody (ILB1-H6), Biotin

          9 篇参考文献
          查看其他100个IL-1 beta抗体

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          Cite IL-1 beta Monoclonal Antibody (ILB1-H6), Biotin

          • 抗体检测数据 (1)
          IL-1 beta Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.
          IL-1 beta Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.

          图: 1 / 1

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          IL-1 beta Antibody (M420BB) in ELISA

          Sandwich ELISA analysis of Mouse anti-Human IL-1beta biotinylated monoclonal antibody (Product # M420BB) was performed using the Thermo Scientific ELISA kit EH2IL1B by loading 50 µL per well of Human IL-1beta recombinant protein (Product # SIL1B) in quadruplicate at 400, 160, 64, 25.6, 10.24 and 0 pg/mL across a 5 µg/mL Mouse anti-Human IL-1beta (Product # M421B) pre-coated plate and incubated for 3 hours at room temperature. The plate was washed then incubated with 50 µL per well of Mouse anti-Human IL-1beta biotinylated antibody (Product... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          IL-1 beta Antibody in ELISA (ELISA)

          产品信息

          M420BB

          应用
          建议稀释比
          已发表文章

          酶联免疫吸附实验 (ELISA)

          Assay-dependent
          查看9篇已发表文章 9篇已发表文章
          产品规格

          种属反应

          Human

          已发表种属

          Human

          宿主/亚型

          Mouse / IgG1, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          ILB1-H6

          抗原

          Recombinant human IL-1 beta

          偶联物

          Biotin Biotin Biotin

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          Protein G

          保存液

          PBS with 4% BSA

          内含物

          proprietary Preservative

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_223565

          产品详细信息

          M420BB targets IL-1 beta in ELISA applications and shows reactivity with Human samples.



          The M420BB immunogen is recombinant human IL-1 beta.



          M420BB detects IL-1 beta which has a predicted molecular weight of approximately 31 kDa.



          The M420BB IL1b antibody (biotinylated conjugate of clone ILB1-H6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M421B (clone ILB1-H67). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.



          Biotinylated antibody M420BB (clone ILB1-H6) and antibody M421B (clone ILB1-H67) have successfully been used in combination with recombinant IL1 beta protein SIL1B in ELISA applications.

          靶标信息

          Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin 1, beta; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; pro-interleukin-1-beta

          查看更多 收起

          基因别名: IL-1; IL1-BETA; IL1B; IL1F2

          查看更多 收起

          UniProt ID: (Human) P01584

          查看更多 收起

          Entrez Gene ID: (Human) 3553

          查看更多 收起

          功能
          cytokine activity interleukin-1 receptor binding protein domain specific binding interleukin superfamily
          参与通路
          negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity fever generation response to hypoxia positive regulation of protein phosphorylation chronic inflammatory response to antigenic stimulus positive regulation of T cell mediated immunity purine nucleobase metabolic process apoptotic process inflammatory response signal transduction positive regulation of cytosolic calcium ion concentration cell-cell signaling embryo implantation aging memory estrogen metabolic process negative regulation of cell proliferation glycoprotein metabolic process response to heat response to ozone response to gamma radiation positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of glucose transport negative regulation of glutamate secretion smooth muscle adaptation cytokine-mediated signaling pathway pentacyclic triterpenoid metabolic process hyaluronan biosynthetic process neutrophil chemotaxis polyketide metabolic process ovulation sequestering of triglyceride positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of prostaglandin secretion response to estradiol interleukin-1 beta production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of immature T cell proliferation in thymus positive regulation of histone phosphorylation response to ATP response to vitamin D response to L-ascorbic acid positive regulation of heterotypic cell-cell adhesion positive regulation of histone acetylation social behavior ectopic germ cell programmed cell death positive regulation of myosin light chain kinase activity response to stilbenoid cellular response to drug response to diuretic response to statin wound healing positive regulation of T cell proliferation positive regulation of NF-kappaB import into nucleus positive regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling response to morphine negative regulation of MAP kinase activity response to peptide hormone protein kinase B signaling positive regulation of JUN kinase activity positive regulation of chemokine biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of nitric oxide biosynthetic process response to ethanol negative regulation of neuron differentiation positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway positive regulation of protein export from nucleus positive regulation of astrocyte differentiation positive regulation of phagocytosis regulation of insulin secretion negative regulation of lipid catabolic process positive regulation of lipid catabolic process regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of cell division positive regulation of cell adhesion molecule production positive regulation of calcidiol 1-monooxygenase activity negative regulation of adiponectin secretion positive regulation of ERK1 and ERK2 cascade monocyte aggregation cellular response to antibiotic cellular response to mechanical stimulus cellular response to organic substance cellular response to glucose stimulus cellular response to fatty acid cellular response to organic cyclic compound cellular response to methotrexate response to dexamethasone positive regulation of monocyte chemotactic protein-1 production positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand regulation of establishment of endothelial barrier negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation positive regulation of interleukin-6 secretion negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.
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