Invitrogen

IL-10 Monoclonal Antibody (12G8), Biotin

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产品信息

M011B

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1000
-

流式细胞分析 (Flow)

-
查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent
查看17篇已发表文章 17篇已发表文章
产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

12G8

抗原

Recombinant human IL-10 (COS cell-derived)

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein G

保存液

PBS with 4% BSA

内含物

proprietary preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_223553

产品详细信息

M011B targets IL-10 in ELISA and Western blot applications and shows reactivity with human samples.



The M011B immunogen is recombinant human IL-10 (COS cell-derived). The original parent clone is 12G8 and has been subcloned as 2553.2.4.



M011B detects IL-10 which has a predicted molecular weight of approximately 19 kDa.



The M011B IL10 antibody (biotinylated conjugate of clone 12G8) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M010 (clone 9D7). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.



Biotinylated antibody M011B (clone 12G8) and antibody M010 (clone 9D7) have successfully been used in combination with recombinant IL10 protein SIL10 in ELISA applications.



This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

靶标信息

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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基因别名: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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功能
cytokine activity interleukin-10 receptor binding protein binding growth factor activity
参与通路
response to molecule of bacterial origin negative regulation of cytokine secretion involved in immune response negative regulation of chronic inflammatory response to antigenic stimulus positive regulation of B cell apoptotic process inflammatory response cytoplasmic sequestering of NF-kappaB cell-cell signaling aging regulation of gene expression response to activity response to inactivity hemopoiesis B cell differentiation leukocyte chemotaxis negative regulation of myeloid dendritic cell activation negative regulation of B cell proliferation negative regulation of interferon-gamma production negative regulation of interleukin-1 production negative regulation of interleukin-12 production negative regulation of interleukin-18 production negative regulation of interleukin-6 production negative regulation of interleukin-8 production negative regulation of tumor necrosis factor production receptor biosynthetic process response to insulin response to carbon monoxide cellular response to hepatocyte growth factor stimulus type 2 immune response B cell proliferation negative regulation of T cell proliferation response to drug negative regulation of tumor necrosis factor biosynthetic process defense response to bacterium defense response to protozoan negative regulation of apoptotic process negative regulation of growth of symbiont in host negative regulation of nitric oxide biosynthetic process regulation of isotype switching negative regulation of MHC class II biosynthetic process positive regulation of MHC class II biosynthetic process negative regulation of interferon-alpha biosynthetic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade positive regulation of cytokine secretion negative regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity response to glucocorticoid regulation of sensory perception of pain negative regulation of cytokine activity branching involved in labyrinthine layer morphogenesis cellular response to lipopolysaccharide cellular response to estradiol stimulus negative regulation of chemokine (C-C motif) ligand 5 production regulation of complement-dependent cytotoxicity negative regulation of endothelial cell apoptotic process
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