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Invitrogen
IL-12 p40 Polyclonal Antibody
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FIGURE: 1 / 1
IL-12 p40 Antibody (PA5-79461) in WB
Western blot analysis of IL-12 p40 in Lane 1: mouse NIH3T3 cell lysate, Lane 2: human A549 cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with IL-12 p40 polyclonal antibody (Product # PA5-79461) at a dilution of 0.5 µg/mL (overnight at 4°C), followed b... View More
Product Details
PA5-79461
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human IL12B recombinant protein (Position: I23-E253).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse NIH3T3 cell, human A549 cell.
Target Information
IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL12 p40; IL12, subunit p40; IL12A; Interleukin; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; Interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF1; NKSF2; RP23-388G23.1
Gene Aliases: CLMF; CLMF2; IL-12B; Il-12p40; IL12B; Il12p40; IMD28; IMD29; NKSF; NKSF2; p40
UniProt ID: (Human) P29460, (Mouse) P43432
Entrez Gene ID: (Human) 3593, (Mouse) 16160
cytokine receptor activity
cytokine activity
interleukin-12 receptor binding
protein binding
growth factor activity
interleukin-12 alpha subunit binding
identical protein binding
protein homodimerization activity
interleukin-23 receptor binding
protein heterodimerization activity
transmembrane signal receptor
positive regulation of T cell mediated cytotoxicity
positive regulation of defense response to virus by host
natural killer cell activation involved in immune response
positive regulation of T-helper 1 type immune response
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
negative regulation of inflammatory response to antigenic stimulus
cell cycle arrest
response to UV-B
positive regulation of activation of JAK2 kinase activity
cell migration
cytokine-mediated signaling pathway
sensory perception of pain
sexual reproduction
natural killer cell activation
negative regulation of interleukin-10 production
negative regulation of interleukin-17 production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-17 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell activation
positive regulation of natural killer cell proliferation
positive regulation of mononuclear cell proliferation
positive regulation of tissue remodeling
positive regulation of smooth muscle cell apoptotic process
regulation of cytokine biosynthetic process
T-helper 1 type immune response
T-helper cell differentiation
interferon-gamma biosynthetic process
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
positive regulation of NF-kappaB import into nucleus
regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat4 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
defense response to protozoan
positive regulation of memory T cell differentiation
negative regulation of growth of symbiont in host
positive regulation of interferon-gamma biosynthetic process
positive regulation of osteoclast differentiation
positive regulation of cell adhesion
negative regulation of smooth muscle cell proliferation
positive regulation of lymphocyte proliferation
positive regulation of inflammatory response
defense response to Gram-negative bacterium
positive regulation of NK T cell activation
positive regulation of NK T cell proliferation
defense response to virus
cellular response to lipopolysaccharide
cellular response to interferon-gamma
positive regulation of T-helper 17 type immune response
positive regulation of T-helper 17 cell lineage commitment
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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