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Invitrogen
IL-17A Monoclonal Antibody (eBio17CK15A5), Functional Grade, eBioscience™
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IL-17A Antibody (16-7175-85) in ELISA
Cell reports 2021 -
Figure 4. MHC class II + ILC3s limit airway Th17 cell responses to microbes upon HDM exposure (A) Representative plots and quantitative graphs of the frequency and number of Th17 cells in lung parenchyma and MedLN from H2-Ab1 fl/fl control mice and MHC class II DeltaILC3 mice after exposure to HDM or PBS. Cells were gated on live CD45 + CD19 - CD3/CD5 + CD4 + (data pooled from 4 independent assays, n = 6). ... View More
产品信息
16-7175-85
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
C. elegans,
Human,
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBio17CK15A5
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The eBio17CK15A5 antibody reacts with mouse and rat IL-17A. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.
IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.
Applications Reported: The eBio17CK15A5 antibody has been reported useful for mouse IL-17A ELISA and ELISPOT capture.
Applications Tested: The Functional Grade Purified eBio17CK15A5 antibody has been tested by LAL assay to verify low endotoxin level and has been tested for mouse IL-17A ELISPOT and ELISA capture.
The Functional Grade Purified eBio17CK15A5 antibody has been tested as the capture antibody in a sandwich ELISA for measurement of mouse IL-17A protein levels, in combination with the biotinylated eBio17B7 antibody (13-7177) for detection and recombinant mouse IL-17A (14-8171) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1.0 - 4.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; il 17; IL-17; ILN; Interleukin; interleukin 17; Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin17A; R-IL-17-A
基因别名: CTLA-8; Ctla8; IL-17; IL-17A; Il17; Il17a
UniProt ID: (Mouse) Q62386
Entrez Gene ID: (Mouse) 16171, (Rat) 301289
inflammatory response
positive regulation of necrotic cell death
positive regulation of interleukin-23 production
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
cellular response to interleukin-1
cellular response to glucocorticoid stimulus
fibroblast activation
positive regulation of cytokine production involved in inflammatory response
response to amino acid
