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Invitrogen
IL-2 Monoclonal Antibody (MQ1-17H12), eBioscience™
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图: 1 / 14
IL-2 Antibody (14-7029-81) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD4 Monoclonal Antibody, APC (Product # 17-0049-42) and 0.125 µg IL-2 Monoclonal Antibody followed by IgG2a Monoclonal Antibody, PE (Product # 12-4817-82). Cells in the lymphocyte gate were used for analysis.
产品信息
14-7029-81
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
C. elegans,
Cynomolgus monkey,
Human,
Mouse,
Non-human primate,
Rhesus monkey
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
MQ1-17H12
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.
Applications Reported: The MQ1-17H12 antibody has been reported for use as capture antibody for ELISA and for intracellular staining for flow cytometric analysis. Fluorochrome conjugated MQ1-17H12 antibody is recommended for use in intracellular staining for flow cytometry.
Applications Tested: The MQ1-17H12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-2 (IL-2) in combination with the biotin anti-human IL-2 poly (13-7028) antibody for detection and recombinant human IL-2 (14-8029) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
基因别名: IL-2; IL2; lymphokine; TCGF
UniProt ID: (Human) P60568
Entrez Gene ID: (Human) 3558
MAPK cascade
negative regulation of protein phosphorylation
adaptive immune response
negative regulation of B cell apoptotic process
immune response
cell adhesion
positive regulation of cytosolic calcium ion concentration
protein kinase C-activating G-protein coupled receptor signaling pathway
cell-cell signaling
positive regulation of cell proliferation
natural killer cell activation
T cell differentiation
positive regulation of cell growth
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of tissue remodeling
positive regulation of activated T cell proliferation
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of apoptotic process
positive regulation of GTPase activity
response to ethanol
positive regulation of regulatory T cell differentiation
negative regulation of heart contraction
positive regulation of transcription from RNA polymerase II promoter
regulation of T cell homeostatic proliferation
positive regulation of isotype switching to IgG isotypes
negative regulation of lymphocyte proliferation
negative regulation of inflammatory response
positive regulation of inflammatory response
positive regulation of immunoglobulin secretion
positive regulation of dendritic spine development
extrinsic apoptotic signaling pathway in absence of ligand
