Rockland

IL-21 Polyclonal Antibody

Name: IL-21 Polyclonal Antibody
Brand: Rockland
SKU: 200-401-B53

查看其他38个IL-21抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

图: 1 / 1

IL-21 Antibody (200-401-B53) in WB

Western blot using Rocklands anti-bovine IL-21 antibody shows detection of recombinant bovine IL-21 at 15.1kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Rocklands, Inc. Anti-bovine IL-21. After washing, membrane was probed with Dylight ™ 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody (611-743-127).

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Immunogen image

Immunogen Image IL-21 Polyclonal Antibody

产品信息

200-401-B53

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

酶联免疫吸附实验 (ELISA)

1:6,000

免疫沉淀 (IP)

1 µg/mL

产品规格

种属反应

Bovine, Horse, Pig

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Protein A

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined.

靶标信息

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

仅用于科研。不用于诊断过程。未经明确授权不得转售。