Invitrogen

IL-23 p19 Antibody Cocktail, eBioscience™

Name: IL-23 p19 Antibody Cocktail, eBioscience\u2122
Brand: Invitrogen
SKU: 14-7238-85

3 篇参考文献

查看其他45个IL-23 p19抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

产品信息

14-7238-85

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫组化（冰冻） (IHC (F))

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酶联免疫吸附实验 (ELISA)

2.5-8 µg/mL

功能检测 (Functional)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Cocktail

类型

Antibody

克隆号

eBio473P19 (eBio11P19, eBio25P19)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

500 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_468464

产品详细信息

Description: The antibody eBio473P19 reacts with the p19 subunit of human IL-23. The eBio473P19 antibody was generated from immunization with authentic, insect cell-expressed, recombinant human IL-23 heterodimer.

The use of a p19-specific capture antibody and a p40-specific detection antibody yields a human IL-23 sandwich ELISA exquisitely specific for human IL-23. IL-12 p40 monomer and IL-12 p70 were run in the assay at 200 ng/mL with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was also run in the IL-23 ELISA at 100 ng/mL with no cross reactivity observed. The assay has been validated by specific detection of significant levels of native human IL-23 protein in supernatants from a variety of different activated dendritic cell populations.

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-g. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-g production by naive and memory T cells, as compared to IL-12. IL-23-dependent, IL-17-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. More recently, de novo differentiation of Th-17 cells in the absence of IL-23 has been demonstrated by treatment of naive CD4 cells with TGF beta1 and IL-6.

Applications Reported: This eBio473P19 antibody has been reported for use in immunoblotting (WB), and ELISA.

Applications Tested: The eBio473P19 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-23 (p19p40) protein levels in combination with the biotinylated (p40-specific) C8.6 antibody (13-7129) for detection and recombinant human IL-23 (14-8239) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2.5-8.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant IL-23 standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate. TMB, rather than ABTS, should be used as the substrate.The antibody eBio473p19 also works in immunoblotting under both reducing and nonreducing conditions at 2 µg/mL.

*eBio473P19 is a cocktail of the two clones, eBio11P19 and eBio25P19 at 0.5 mg/mL each. Please contact Tech Support for more information regarding the concentration.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: il 23; IL 23 A; Il-12b; Il-12p40; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12B; Il12p40; il23; ILN; Interleukin; interleukin 12B; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; MGC79388; p40; RP23-388G23.1

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基因别名: IL-23; IL-23A; IL23A; IL23P19; P19; SGRF; UNQ2498/PRO5798

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UniProt ID: (Human) Q9NPF7

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Entrez Gene ID: (Human) 51561

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功能

cytokine activity protein binding interleukin-23 receptor binding

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