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Rockland
IL-27 Polyclonal Antibody
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FIGURE: 1 / 2
IL-27 Antibody (600-401-BW1) in ICC/IF
Immunocytochemistry of IL-27 antibody. Cell Type: EL4 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: IL-27 antibody at 10 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: IL-27 is secreted. Staining: IL-27 is stained with hematoxylin purple nuclear counterstain.
Product Details
600-401-BW1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-IL-27 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the N-terminus of human IL-27.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine receptor; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; Epstein-Barr virus-induced gene 3 protein homolog; IL-27 p28 subunit; IL-27 subunit alpha; IL-27 subunit beta; IL-27-A; IL-27B; IL27 p28; IL27 subunit; IL27-A; IL35 subunit; ILN; Interleukin; interleukin 30; Interleukin-27 subunit alpha; Interleukin-27 subunit beta; Interleukin-30; M-IL-27 (p28); MGC71873; p28
Gene Aliases: EBI-3; EBI3; IL-27; IL-27A; IL-27B; IL-27p28; IL27; IL27A; IL27B; IL27p28; IL30; p28
UniProt ID: (Human) Q14213, (Mouse) O35228, (Human) Q8NEV9, (Mouse) Q8K3I6
Entrez Gene ID: (Human) 10148, (Mouse) 50498, (Human) 246778, (Mouse) 246779
humoral immune response
cytokine-mediated signaling pathway
T-helper 1 type immune response
T cell proliferation
positive regulation of interferon-gamma biosynthetic process
positive regulation of alpha-beta T cell proliferation
inflammatory response
response to bacterium
regulation of T cell proliferation
innate immune response
regulation of T-helper 1 cell differentiation
regulation of defense response to virus
immune system process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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