Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
IL-33 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 2
IL-33 Antibody (PA5-96929) in WB
Western blot analysis of IL-33 in extracts of various cell lines. Samples were incubated with IL-33 Polyclonal antibody (Product # PA5-96929) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-96929
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 110-190 of human IL33 (NP_2542741)
偶联物
形式
Liquid
浓度
1.06 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: DSSITGISPI TEYLASLSTY NDQSITFALE DESYEIYVED LKKDEKKDKV LLSYYESQHP SNESGDGVDG KMLMVTLSPT K
Positive Samples: NCI-H460, SGC-7901, MCF7, Rat kidney; Cellular Location: Chromosome, Cytoplasmic vesicle, Nucleus, secretory vesicle
靶标信息
IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DVS27-related protein; Fit-1; Fos-responsive gene 1 protein; growth stimulation-expressed; H-IL-33; homolog of mouse growth stimulation-expressed; IL-1F11; IL-33; ILN; Interleukin; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin-1 family member 11; Interleukin-1 receptor-like 1; Interleukin-33; Interleukin-33 receptor alpha chain; Interleukin33; Lymphocyte antigen 84; NF-HEV; nuclear factor for high endothelial venules; Nuclear factor from high endothelial venules; Protein ST2; Protein T1; RP11-575C20.2
基因别名: 9230117N10Rik; C9orf26; DER4; DVS27; FIT-1; FIT1; Il-33; IL1F11; IL1RL1; IL33; IL33R; Ly84; NF-HEV; NFEHEV; NFHEV; RGD1311155; ST2; St2-rs1; ST2L; ST2V; Ste2; T1; T1/ST2
UniProt ID: (Human) Q01638, (Human) O95760, (Mouse) P14719, (Rat) Q62611, (Mouse) Q8BVZ5, (Rat) Q66H70
Entrez Gene ID: (Human) 9173, (Human) 90865, (Mouse) 17082, (Rat) 25556, (Mouse) 77125, (Rat) 361749
negative regulation of T-helper 1 type immune response
immune response
signal transduction
negative regulation of cell proliferation
negative regulation of interferon-gamma production
positive regulation of interleukin-5 production
interleukin-33-mediated signaling pathway
positive regulation of macrophage activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of inflammatory response
positive regulation of chemokine secretion
cellular response to peptide
negative regulation of leukocyte migration
positive regulation of type 2 immune response
transcription, DNA-templated
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of interleukin-13 production
positive regulation of interleukin-4 production
positive regulation of interleukin-6 production
type 2 immune response
positive regulation of transcription from RNA polymerase II promoter
positive regulation of immunoglobulin secretion
negative regulation of immunoglobulin secretion
defense response to virus
extrinsic apoptotic signaling pathway
cytokine-mediated signaling pathway
positive regulation of gene expression
positive regulation of cytokine production
