Invitrogen

IL-4 Monoclonal Antibody (11B11), Alexa Fluor™ 488, eBioscience™

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产品信息

53-7041-82

应用
建议稀释比
已发表文章

免疫组化 (IHC)

-
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流式细胞分析 (Flow)

1 µg/test
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酶联免疫吸附实验 (ELISA)

-
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产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG1, kappa

推荐的同型对照

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

11B11

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱 spectra

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_469922

产品详细信息

Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

Applications Reported: This 11B11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 11B11 antibody has been tested by intracellular staining and flow cytometric analysis of Th2 polarized and stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

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基因别名: BSF-1; Il-4; Il4

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UniProt ID: (Mouse) P07750

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Entrez Gene ID: (Mouse) 16189

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功能
cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily
参与通路
microglial cell activation positive regulation of protein phosphorylation innate immune response in mucosa positive regulation of defense response to virus by host T-helper 1 cell lineage commitment positive regulation of immunoglobulin production negative regulation of acute inflammatory response negative regulation of chronic inflammatory response immune response cholesterol metabolic process positive regulation of cell proliferation regulation of proton transport negative regulation of epithelial cell migration positive regulation of B cell proliferation B cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-13 production T-helper 2 cell cytokine production positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation positive regulation of tyrosine phosphorylation of Stat5 protein defense response to protozoan negative regulation of macrophage activation positive regulation of mast cell degranulation negative regulation of nitric oxide biosynthetic process T-helper 2 cell differentiation positive regulation of chemokine biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of T cell differentiation negative regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgE isotypes positive regulation of isotype switching to IgG isotypes regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response negative regulation of T cell activation positive regulation of B cell activation positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of mononuclear cell migration dendritic cell differentiation extrinsic apoptotic signaling pathway in absence of ligand positive regulation of myoblast fusion negative regulation of complement-dependent cytotoxicity negative regulation of T-helper 17 cell differentiation negative regulation of endothelial cell apoptotic process positive regulation of eosinophil chemotaxis negative regulation of extrinsic apoptotic signaling pathway
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