Invitrogen

IL-4 Monoclonal Antibody (1D11)

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产品信息

MM450C

应用
建议稀释比
已发表文章

免疫印迹 (WB)

Assay-dependent
-

免疫细胞化学 (ICC/IF)

-
查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

-
查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent
查看26篇已发表文章 26篇已发表文章

中和实验 (Neu)

-
查看1篇已发表文章 1篇已发表文章
产品规格

种属反应

Mouse

已发表种属

Mouse, Rat

宿主/亚型

Rat / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

1D11

抗原

Recombinant mouse IL-4

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_223530

产品详细信息

MM450C targets IL-4 in ELISA, and WB applications and shows reactivity with mouse samples. Does not bind to human IL-4.



The MM450C immunogen is recombinant mouse IL-4.



MM450C detects IL-4 which has a predicted molecular weight of approximately 14 kDa.



This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).



The MM450C mouse IL4 antibody (clone 1D11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM450DB (biotinylated conjugate of clone 24G2). Typical dilutions for sandwich ELISA include 1-5 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.



Antibody MM450C (clone 1D11) and biotinylated antibody MM450DB (clone 24G2) have successfully been used in combination with recombinant mouse IL4 protein SMIL4 in ELISA applications.



This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

靶标信息

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

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基因别名: BSF-1; Il-4; Il4

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UniProt ID: (Mouse) P07750

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Entrez Gene ID: (Mouse) 16189

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功能
cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily
参与通路
microglial cell activation positive regulation of protein phosphorylation innate immune response in mucosa positive regulation of defense response to virus by host T-helper 1 cell lineage commitment positive regulation of immunoglobulin production negative regulation of acute inflammatory response negative regulation of chronic inflammatory response immune response cholesterol metabolic process positive regulation of cell proliferation regulation of proton transport negative regulation of epithelial cell migration positive regulation of B cell proliferation B cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-13 production T-helper 2 cell cytokine production positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation positive regulation of tyrosine phosphorylation of Stat5 protein defense response to protozoan negative regulation of macrophage activation positive regulation of mast cell degranulation negative regulation of nitric oxide biosynthetic process T-helper 2 cell differentiation positive regulation of chemokine biosynthetic process positive regulation of MHC class II biosynthetic process positive regulation of T cell differentiation negative regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgE isotypes positive regulation of isotype switching to IgG isotypes regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response negative regulation of T cell activation positive regulation of B cell activation positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of mononuclear cell migration dendritic cell differentiation extrinsic apoptotic signaling pathway in absence of ligand positive regulation of myoblast fusion negative regulation of complement-dependent cytotoxicity negative regulation of T-helper 17 cell differentiation negative regulation of endothelial cell apoptotic process positive regulation of eosinophil chemotaxis negative regulation of extrinsic apoptotic signaling pathway
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