Invitrogen

IL-4 Monoclonal Antibody (8D4-8), APC, eBioscience™

Name: IL-4 Monoclonal Antibody (8D4-8), APC, eBioscience\u2122
Brand: Invitrogen
SKU: 17-7049-82

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13 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

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IL-4 Antibody (17-7049-82) in Flow

Frontiers in cell and developmental biology 2022 - FIGURE 2 Cytokine-producing T cells from peripheral blood. PBMCs isolated from moderate-to-severe plaque psoriasis patients and healthy controls were stimulated with Cell Stimulation Cocktail for 5 h. The IFN-gamma-, IL-4- and IL-17-producing T cells were determined by intracellular staining and flow cytometry analysis. (A) The proportion of IFN-gamma-,IL-4- and IL-17-producing CD4 + T cells in psoriasis pa... View More

Product Image Gallery

IL-4 Antibody — Figure 6. piR30840 regulates the development of Th2 lymphocytes. ( A and B ) Human CD4 naive cells were cultured in a conditioned medium for Th2 development and then transfected with an s30840 (A) or piR30840 inhibitor (B). IL-4 expression was detected by qRT-PCR and western blotting analyses. P < 0.01. The data represents three independent experiments. ( C ) Detection of IL-4 expression in human PBMCs isolated from humanized NOG mice. IL-4 expression was detected by qRT-PCR analysis. The data was expressed as the mean of three independent experiments. Wilcoxon test was used to calculate the P -value. P < 0.05. ( D ) CD4 naive cells were cultured in the conditioned medium for Th2 development and transfected with s30840 or control. After 7 days, the Th2 T-lymphocytes (intracellular stained as IL-4 + IFN-gamma - ) were detected with FACS analysis. The data was shown as a mean of six independent experiments. P < 0.01. ( E ) The CD4 naive cells were cultured in a conditioned medium for Th2 development with or without the transfection of inhibitor. We adoptively transferred the naive cells into the irradiated NOG mice which were first humanized. After 7 days, Th2 T-lymphocytes (intracellularly stained as IL-4 + IFNgamma - ) were detected by using FACS analysis. The data were expressed as the mean of six independent experiments. Statistical significance between two samples was determined by using the student's t -test. P < 0.01. {TM}

产品信息

17-7049-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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产品规格

种属反应

Human

已发表种属

Human, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

8D4-8

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_469498

产品详细信息

Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported: The 8D4-8 antibody has been reported for use as a capture antibody for human IL-4 ELISA and for intracellular staining for flow cytometric analysis.

Applications Tested: This 8D4-8 antibody has been tested in intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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基因别名: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112

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Entrez Gene ID: (Human) 3565

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功能

cytokine activity interleukin-4 receptor binding protein binding growth factor activity interleukin superfamily

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