Invitrogen

IL-6 Monoclonal Antibody (7IL6), Biotin

Name: IL-6 Monoclonal Antibody (7IL6), Biotin
Brand: Invitrogen
SKU: M621B

高级验证

6个已发表图片

22 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

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IL-6 Antibody (M621B) in ELISA

Sandwich ELISA analysis of human IL-6 was performed using a Human IL-6 Colorimetric ELISA kit (Product # EH2IL6) by loading 50 µL per well of a mouse anti-human IL-6 biotinylated antibody (Product # M621B) at a concentration of 0.035 µg/mL and 50 µL of Human IL-6 Recombinant Protein (Product # SIL6) at 400, 160, 64, 25.6, 10.24, and 0 pg/mL (both in quadruplicate) across a 4 µg/mL mouse anti-human IL-6 (Product # M620) pre-coated plate. The plate was incubated for 2 hours at room temperature, washed, and incubated with 100 µL per well of S... View More

Product Image Gallery

IL-6 Antibody — Figure 7. Effect of Res treatment on miR-9, NF-kappaB1, IL-6, MMP-13, caspase-3 expression in mouse chondrocytes. The relative (A) miR-9 and (B) NF-kappaB1 mRNA expression levels were determined by RT-qPCR in chondrocytes following treatment with 0, 15 or 30 mM Res for 48 h. (C) The relative NF-kappaB1, IL-6, MMP-13 and caspase-3 protein expression levels were determined by western blot analysis in chondrocytes following treatment with 0, 15 or 30 mM Res for 48 h. Quantification of (D) NF-kappaB1, (E) IL-6, (F) MMP-13 and (G) caspase-3 protein expression. n=3. *P<0.05 vs. control group. miR, microRNA; NF-kappaB1, nuclear factor kappa B subunit 1; IL-6, interleukin-6; MMP-13, matrix metallopeptidase 13; Res, Resveratrol. {TM}

产品信息

M621B

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

Assay-dependent

查看18篇已发表文章 18篇已发表文章

产品规格

种属反应

Human

已发表种属

Cynomolgus monkey, Dog, Human, Mouse, Pig

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

7IL6

抗原

Recombinant human IL-6.

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein G

保存液

PBS with 4% BSA

内含物

proprietary Preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_223577

产品详细信息

M621B targets IL-6 in ELISA and WB applications and shows reactivity with Human samples.

The M621B immunogen is recombinant human IL-6.

M621B detects IL-6 which has a predicted molecular weight of approximately 22 kDa.

The M621B IL6 antibody (biotinylated conjugate of clone 7IL6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M620 (clone 5IL6). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M621B (clone 7IL6) and antibody M620 (clone 5IL6) have successfully been used in combination with recombinant IL6 protein SIL6 in ELISA applications.

靶标信息

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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基因别名: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231

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Entrez Gene ID: (Human) 3569

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功能

cytokine activity interleukin-6 receptor binding protein binding growth factor activity

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