Invitrogen

IL-6 Monoclonal Antibody (MQ2-39C3), Biotin, eBioscience™

Name: IL-6 Monoclonal Antibody (MQ2-39C3), Biotin, eBioscience\u2122
Brand: Invitrogen
SKU: 13-7068-81

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13 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

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IL-6 Antibody (13-7068-81) in ELISA

Frontiers in cellular and infection microbiology 2021 - Figure 4 Host response to P. aeruginosa LESB strains in vitro . (A) Human bronchial epithelial cells (HBE) were infected with P. aeruginosa LESB58 or LESB65 at a multiplicity of infection (MOI) of 1, 5, 10, or 100 for 18 h. Amount of LDH in host-cell supernatants was quantified for determination of cytotoxicity. (B) At MOI = 10, P. aeruginosa LESB65 took 48 h to elicit cytotoxicity similar to that caused in... View More

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IL-6 Antibody — FIGURE 5. TLR blocking experiments. 200,000 human PBMC cells were stimulated with GMMA from different strains. After a 4-h incubation, IL-6 release was measured by ELISA and plotted as average of duplicates with standard deviation. A, IL-6 release after stimulation with 0.0001-1000 ng/ml (10-fold steps) of GMMA from different strains. The rectangle highlights the concentration of GMMA used in blocking experiments. B-E, cells were incubated with 25 mug/ml anti-TLR4 ( dark red graphs ), 15 mug/ml anti-TLR2 ( green graphs ), or both ( violet graphs ) for 30 min before exposure to 1 or 10 ng of GMMA from Ss -p - OAg Delta msbB ( B ); Sf 2a -p - OAg Delta msbB ( C ); Ss -p - OAg Delta htrB ( D ); and Sf 2a -p - OAg Delta htrB ( E ). The graphs from experiments without blocking are shown in the same color as in A . A representative result of three independent experiments is shown. {TM}

产品信息

13-7068-81

应用

建议稀释比

已发表文章

酶联免疫吸附实验 (ELISA)

0.5-2 µg/mL

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肽阵列 (Array)

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

MQ2-39C3

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466912

产品详细信息

Description: The MQ2-39C3 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: MQ2-39C3 has been reported for use in detection of human IL-6 by ELISA and immunoblotting (WB).

Applications Tested: The MQ2-39C3 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-6 (IL-6) in combination with the purified MQ2-13A5 (14-7069) antibody for capture and recombinant human IL-6 (14-8069) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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基因别名: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231

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Entrez Gene ID: (Human) 3569

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功能

cytokine activity interleukin-6 receptor binding protein binding growth factor activity

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