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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
AA Sequence of recombinant protein: MECHIKDKEG KAYESVLMIS IDELDKMTGT DSNCPNNEPN FFRKHVCDDT KEAAFLNRAA RKLKQFLKMN ISEEFNVHLL TVSQGTQTLV NCTSKEEKNV KEQKKNDACF LKRLLREIKT CWNKILKGSI.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-7. Anti-Murine IL-7-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine IL-7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-7 (500-P57) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-7.
Western Blot: To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P57BT-1MG will be provided as 2 x 500 µg
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: H-IL-7; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; ILN; Interleukin; interleukin 7 receptor alpha chain; interleukin-7 receptor precursor; Interleukin7; M-IL-7; unnamed protein product
基因别名: A630026I06Rik; CD127; hlb368; Il-7; IL-7Ralpha
UniProt ID: (Mouse) P10168, (Mouse) P16872
Entrez Gene ID: (Mouse) 16196, (Mouse) 16197