Invitrogen

IL-9 Monoclonal Antibody (RM9A4), eFluor™ 660, eBioscience™

Name: IL-9 Monoclonal Antibody (RM9A4), eFluor\u2122 660, eBioscience\u2122
Brand: Invitrogen
SKU: 50-8091-82

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2 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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IL-9 Antibody (50-8091-82) in Flow

BALB/c splenocytes were cultured with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82), Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82), Mouse IL-4 Recombinant Protein (Product # 14-8041-80), and Human TGF beta 1 Recombinant Protein (Product # 14-8348-62) for 3 days, then cultured with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) for an additional 5 hours. Cells were stained intracellularly with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.03 µg Anti-M... View More

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产品信息

50-8091-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.06 µg/test

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Mouse

已发表种属

C. elegans

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

RM9A4

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11218680

产品详细信息

Description: This monoclonal RM9A4 antibody reacts with mouse Interleukin-9 (IL-9). IL-9 is a 14 kDa cytokine originally named P40 and identified by its proliferative effects on T cell populations. The receptor, which is a heterodimer of the gamma chain portion of the IL-2 receptor and the IL-9R chain, activates Jak/STAT signaling pathways upon binding its ligand. Since the discovery of IL-9, numerous other functions have been observed. It induces Th17 and Treg differentiation in CD4+ T cells, IgE production in B cells, and the differentiation and proliferation of mast cells. IL-9 expression was initially observed in Th2 cells, but has since been found in Th17, eosinophil, and mast cells. Th9 cells, a newly discovered subset of CD4+ T cells, are characterized by the secretion of large amounts of IL-9 and IL-10. Th9 development is induced by stimulation of undifferentiated CD4+ with IL-4 and TGF beta. Th2 cells can also be driven towards a Th9 phenotype in the presence of TGF beta.

Applications Reported: This RM9A4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This RM9A4 antibody has been tested by intracellular staining followed by flow cytometric analysis of cultured mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.

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