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Invitrogen
IL5RA Polyclonal Antibody
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IL5RA Antibody (PA5-121210) in WB
Western blot analysis of IL5RA in extracts of various cell lines. Samples were incubated with IL5RA Polyclonal antibody (Product # PA5-121210) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-121210
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 20-335 of human IL5RA (NP_783855.1).
偶联物
形式
Liquid
浓度
0.54 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive test controls include: HL-60, B cells, U-87MG, Mouse liver, Mouse lung, Rat lung. The target is usually found in the following locations: Membrane, Single-pass type I membrane protein.
Immunogen sequence: ADLLPDEKIS LLPPVNFTIK VTGLAQVLLQ WKPNPDQEQR NVNLEYQVKI NAPKEDDYET RITESKCVTI LHKGFSASVR TILQNDHSLL ASSWASAELH APPGSPGTSI VNLTCTTNTT EDNYSRLRSY QVSLHCTWLV GTDAPEDTQY FLYYRYGSWT EECQEYSKDT LGRNIACWFP RTFILSKGRD WLAVLVNGSS KHSAIRPFDQ LFALHAIDQI NPPLNVTAEI EGTRLSIQWE KPVSAFPIHC FDYEVKIHNT RNGYLQIEKL MTNAFISIID DLSKYDVQVR AAVSSMCREA GLWSEWSQPI YVGFSR
靶标信息
Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cell replacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membrane-proximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD125; CD125 antigen; CDw125; IL-5 receptor alpha chain; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; Interleukin-5 receptor subunit alpha
基因别名: CD125; CDw125; HSIL5R3; IL5R; IL5RA
UniProt ID: (Human) Q14633, (Mouse) P21183
Entrez Gene ID: (Human) 3568, (Rat) 114103, (Mouse) 16192
MAPK cascade
inflammatory response to antigenic stimulus
signal transduction
cell proliferation
peptidyl-tyrosine phosphorylation
regulation of interleukin-5 production
interleukin-5-mediated signaling pathway
positive regulation of GTPase activity
cytokine-mediated signaling pathway
cellular response to organic substance
