Invitrogen

IQGAP1 Polyclonal Antibody

Name: IQGAP1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95625

查看其他16个IQGAP1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (9)

IQGAP1 Antibody in Immunocytochemistry (ICC/IF)

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IQGAP1 Antibody (PA5-95625) in ICC/IF

Immunocytochemistry analysis of IQGAP1 using anti-IQGAP1 antibody (Product # PA5-95625) . IQGAP1 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-IQGAP1 antibody (Product # PA5-95625) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAP... View More

IQGAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IQGAP1 Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image IQGAP1 Polyclonal Antibody

产品信息

PA5-95625

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human IQGAP1 recombinant protein (D1374-K1657).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2807427

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human placenta tissue, human U-87MG whole cell. IHC: human intestinal cancer tissue, human intestinal cancer tissue, human mammary cancer tissue, human mammary cancer tissue. ICC/IF: U20S cell. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

仅用于科研。不用于诊断过程。未经明确授权不得转售。