Invitrogen

IRAK1 Polyclonal Antibody

Name: IRAK1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-19855
Availability: InStock

高级验证

4个已发表图片

4 篇参考文献

查看其他27个IRAK1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

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IRAK1 Antibody (PA5-19855) in WB

Western blot was performed using Anti-IRAK1 Polyclonal Antibody (Product # PA5-19855) and an 80 kDa band corresponding to IRAK1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), LNCaP (Lane 2), HeLa (Lane 3), PC-3 (Lane 4), K-562 (Lane 5), U-2 OS (Lane 6) and T-47D (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB2100... View More

IRAK1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

IRAK1 Antibody — Figure 5 Analysis of E2 signal transduction. ( a ) Western blotting of A549, MCF-10A, and 293T cell lysates showing IL-1R expression; cytoplasmic and membrane components of the cell lysates were separated. A549 cells were included as a positive control for IL-1R1 expression, and 293T cells were used as a negative control. ( b ) Western blotting of MCF-10A cells showing phospho-IRAK1 (left) and IRAK1 (right) following treatment with 100 ng/ml GST-IL-1beta for 0-30 min. ( c ) Western blotting of MCF-10A cells probed to examine phospho-p38(Thr180/Tyr182) and p38 after adding 100 ng/ml GST-IL-1beta for 0-60 min. ( d ) Western blotting of MCF-10A cells probed to examine phospho-p38 (Thr180/Tyr182) and p38 after adding 1 ng/ml GST-IL-1beta for 0-60 min. ( e ) Western blotting of MCF-10A cells showing phospho-JNK(Thr183/Tyr185) and JNK following treatment with 100 ng/ml GST-IL-1beta for 0-60 min. ( f ) Western blotting of MCF-10A cells showing phospho-IkB (Ser32) and IkB following treatment with 100 ng/ml GST-IL-1beta for 0-30 min. ( g ) MMP-3 activity assay of MCF-10A cells following treatment with 100 ng/ml GST-IL-1beta with or without 1.6 muM NNGH, which was used as an MMP-3 inhibitor. Four independent experiments were performed. Bars represent +/-SD. ( h ) Representative confocal images of MCF-10A cells in a 3D culture treated with 100 ng/ml GST-IL-1beta (second row) or E2 (32 nM, third row) or control (first row) for 7 days to examine the basement membrane via immunofluorescenc {TM}

Immunogen image

Immunogen Image IRAK1 Polyclonal Antibody

产品信息

PA5-19855

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

10 µg/mL

免疫沉淀 (IP)

2-4 µg

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A peptide corresponding to amino acids near the carboxy terminus of human IRAK.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11152551

产品详细信息

A suggested positive control is Hela cell lysate.

PA5-19855 can be used with blocking peptide PEP-0001.

靶标信息

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.

仅用于科研。不用于诊断过程。未经明确授权不得转售。