Invitrogen

IRF3 Recombinant Rabbit Monoclonal Antibody (3H32L10)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 篇参考文献

查看其他48个IRF3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

IRF3 Antibody in Immunocytochemistry (ICC/IF)

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IRF3 Antibody (703682) in ICC/IF

For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous IRF3 using Anti-IRF3 Recombinant Rabbit Monoclonal Antibody (Product # 703682, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows representativ... View More

Product Image Gallery

IRF3 Antibody — Detection of subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of IRF3 using Anti-IRF3 Recombinant Rabbit Monoclonal Antibody (Product # 703682) detects IRF3 in the nucleus of A549 cells transfected with Poly (dA:dT) in comparison to cytoplasmic localization in control cells. {TM}

产品信息

703682

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

3H32L10

抗原

Protein corresponding to human IRF3 [aa1-aa427]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2784599

产品详细信息

This antibody is predicted to react with Monkey, Cat, Pig.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells.

Nucleic acids research

Miglietta G,Russo M,Duardo RC,Capranico G

703682 was used in Immunocytochemistry to identify two G-quadruplex binders as regulators of signaling pathways in cancer cells.

Fri Jul 09 00:00:00 EDT 2021

G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells.

Nucleic acids research

Miglietta G,Russo M,Duardo RC,Capranico G

703682 was used in Immunocytochemistry to identify two G-quadruplex binders as regulators of signaling pathways in cancer cells.

Fri Jul 09 00:00:00 EDT 2021

G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells.

Nucleic acids research

Miglietta G,Russo M,Duardo RC,Capranico G

703682 was used in Immunocytochemistry to identify two G-quadruplex binders as regulators of signaling pathways in cancer cells.

Fri Jul 09 00:00:00 EDT 2021

Engineering Supramolecular Organizing Centers for Optogenetic Control of Innate Immune Responses.

Advanced biology

Tan P,He L,Zhou Y

703682 was used in Immunocytochemistry-immunofluorescence to show that synthetic molecular tools will likely enable optical and user-defined modulation of innate immunity at a high spatiotemporal resolution to facilitate mechanistic studies of distinct modes of innate immune activations and potential intervention of immune disorders and cancer.

Sat May 01 00:00:00 EDT 2021

生物信息学

蛋白别名: Interferon regulatory factor 3; IRF-3; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3

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基因别名: IIAE7; IRF3

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UniProt ID: (Human) Q14653

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Entrez Gene ID: (Human) 3661

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription cofactor activity protein binding identical protein binding protein homodimerization activity winged helix/forkhead transcription factor

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