Invitrogen

IRF7 Recombinant Rat Monoclonal Antibody (RDP4ND4)

Name: IRF7 Recombinant Rat Monoclonal Antibody (RDP4ND4)
Brand: Invitrogen
SKU: 740138T

高级验证

查看其他22个IRF7抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (2)

IRF7 Antibody in Immunocytochemistry (ICC/IF)

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IRF7 Antibody (740138T) in ICC/IF

Immunofluorescence analysis of IRF7 was performed using IRF7 Recombinant Rat Monoclonal Antibody (RDP4ND4) (Product # 740138T) on 70-80% confluent PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with IRF7 Recombinant Rat Monoclonal Antibody (RDP4ND4) (Product #740138T, 1:200) in 0.1% BSA, incubated at 4 degree Celsius overnight, followed by labelling with Donkey anti-Rat IgG (H+L) High... View More

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IRF7 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. IRF7 Recombinant Rat Monoclonal Antibody (RDP4ND4) (Product # 740138T) specifically detects IRF7 expression in THP1 differentiated to M1 macrophage cells (pink histogram) while low expression is seen in THP1 cells (blue histogram). {RE}

产品信息

740138T

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫细胞化学 (ICC/IF)

1:200

流式细胞分析 (Flow)

0.25 µg/test

产品规格

种属反应

Human

宿主/亚型

Rat / IgG2a

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

RDP4ND4

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.

仅用于科研。不用于诊断过程。未经明确授权不得转售。