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Agrisera
IRT1/Iron regulated transporter 1 Polyclonal Antibody
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IRT1/Iron regulated transporter 1 Antibody (AS11-1780) in WB
5 µg of total protein from Arabidopsis thaliana wild type (Col-0) and IRT 1 mutant (irt 1-1) extracted with SDG buffer (62 m M Tris-HCL p H 8.6,2.5% SDS, 2% dithiothreitol, 10% glycerol) were separated on 15% SDS-PAGE and blotted 1 h to nitrocellulose. Blots were blocked with 5% milk in TBST for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5,000 ... View More
产品信息
AS11-1780
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana IRT1 sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, This antibody can be stored in a solution containg 50 % glycerol, final concentration, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
IRT1 encodes an iron-regulated, integral membrane metal uptake transporter that is strongly induced in the outer cell layers of Arabidopsis roots under iron limitation and functions at the plant-soil interface. The IRT1 protein belongs to the ZIP family of metal transporters and is a multi-pass plasma-membrane protein with a conserved ZIP domain; it contains a prominent cytosolic loop implicated in metal sensing/turnover, including a histidine-rich motif and lysine residues that are targets for ubiquitin-mediated regulation. In root epidermal cells (including root hairs), IRT1 dynamically cycles between the plasma membrane and the trans-Golgi network/early endosomes, where constitutive endocytosis and monoubiquitin-dependent trafficking help prevent toxic metal overaccumulation while maintaining iron acquisition. Functionally, IRT1 is the major high-affinity entry route for ferrous iron (Fe2+) from the rhizosphere during iron starvation and can also transport other divalent metals such as manganese, zinc, cobalt, and cadmium, making its abundance and subcellular localization critical determinants of whole-plant metal homeostasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ARABIDOPSIS IRON-REGULATED TRANSPORTER 1; AT4G19690; ATIRT1; iron-regulated transporter 1; T16H5.50; T16H5_50
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