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ISG15 Antibody (703132) in ICC/IF
For immunofluorescence analysis, RAW 264.7 cells were fixed and permeabilized for detection of endogenous ISG15 (Mouse) using Anti-ISG15 (Mouse) Recombinant Rabbit Monoclonal Antibody (Product # 703132, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a... View More
产品信息
703132
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
1H9L21
抗原
Protein corresponding to mouse ISG15 [aa2-aa155]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Rat, Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: interferon, alpha-inducible protein; Interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; Interferon-induced 17 kDa protein; interferon-stimulated protein (15 kDa); interferon-stimulated protein 15; IP17; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15
基因别名: G1p2; IGI15; IP17; Irfp; Isg15; UCRP
UniProt ID: (Mouse) Q64339
Entrez Gene ID: (Mouse) 100038882
modification-dependent protein catabolic process
negative regulation of protein ubiquitination
ISG15-protein conjugation
regulation of interferon-gamma production
response to type I interferon
defense response to bacterium
negative regulation of viral genome replication
positive regulation of erythrocyte differentiation
defense response to virus
