Invitrogen

ISM1 Polyclonal Antibody

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3 篇参考文献

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)
已发表图片 (1)

ISM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ISM1 Antibody (PA5-24968) in IHC (P)

Immunohistochemistry analysis of ISM1 in formalin fixed and paraffin embedded human skin carcinoma. Samples were incubated with ISM1 polyclonal antibody (Product # PA5-24968) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ISM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISM1 Antibody in Western Blot (WB)

ISM1 Antibody in Western Blot (WB)

ISM1 Antibody in Western Blot (WB)

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ISM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ISM1 in formalin fixed and paraffin embedded human skin carcinoma. Samples were incubated with ISM1 polyclonal antibody (Product # PA5-24968) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ISM1 Antibody in Western Blot (WB) — Western blot analysis of ISM1 in human placenta tissue lysate. Samples were incubated in ISM1 polyclonal antibody (Product # PA5-24968) using a dilution of 1:2,000 followed by Secondary Goat anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Lysates/proteins at 20 µg per lane. Predicated band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ISM1 Antibody in Western Blot (WB) — Western blot analysis of ISM1 in human placenta tissue lysate. Samples were incubated with ISM1 polyclonal antibody (Product # PA5-24968) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ISM1 Antibody in Western Blot (WB) — Expression of ISM1 and its receptors in different models of glomerulopathies. Estimation by RT-qPCR ( n = 6): ( a ) ISM1 expression in different models of proteinuria (L-NAME (W0), doxorubicin, puromycin, obesity, and LPS. Values normalized to CTL; ( b ) Expression of GRP78 (left) and integrin-beta5 (right) in doxorubicin, puromycin, and obesity models. *, p < 0.05 vs CTL **, p < 0.01 vs CTL ***, p < 0.001 vs CTL ( c ) Interaction between ISM1 and its GRP78 and integrin alphavbeta5 receptors in cultured podocytes. Immunoprecipitation (IP) of GRP78 and integrin alphavbeta5 using podocyte cells and then, Western blot of ISM1. Negative control with unspecific antibody. The ISM1 interacts with its receptors in podocytes, in basal conditions, ( n = 2).

Immunogen image

ISM1 Polyclonal Antibody

Immunogen Image ISM1 Polyclonal Antibody

产品信息

PA5-24968

应用

建议稀释比

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免疫印迹 (WB)

1:2,000

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:50-1:100

-

免疫组化（冰冻） (IHC (F))

-

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产品规格

种属反应

Human

已发表种属

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 435-464 amino acids of human ISM1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

May repress the transcriptional activity of AP-2 family members, including TFAP2A, TFAP2B and TFAP2C to various extent.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: isthmin 1 homolog; Isthmin-1

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基因别名: bA149I18.1; C20orf82; dJ1077I2.1; ISM; ISM1; Isthmin

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UniProt ID: (Human) B1AKI9

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Entrez Gene ID: (Human) 140862

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