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FIGURE: 1 / 5
ITCH Antibody (PA5-18662) in ICC/IF
Immunocytochemistry analysis of ITCH using ITCH Polyclonal Antibody (Product # PA5-18662) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18662
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (EIKSHDLKPNGGN) corresponding to the internal amino acids of ITCH (aa 714-727).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with canine, mouse and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIP4; atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; E3 ubiquitin-protein ligase Itchy homolog; HECT-type E3 ubiquitin transferase Itchy homolog; Itch; itchy E3 ubiquitin protein ligase homolog; NAPP1; Nedd 4 like ubiquitin protein ligase; NFE2-associated polypeptide 1; unnamed protein product
Gene Aliases: ADMFD; AIF4; AIP4; ITCH; NAPP1
UniProt ID: (Human) Q96J02
Entrez Gene ID: (Human) 83737
ubiquitin-protein transferase activity
protein binding
transferase activity
ligase activity
ubiquitin-like protein transferase activity
ubiquitin-ubiquitin ligase activity
ribonucleoprotein complex binding
ubiquitin-like protein ligase binding
CXCR chemokine receptor binding
ubiquitin protein ligase activity
arrestin family protein binding
protein polyubiquitination
regulation of cell growth
immune system process
positive regulation of T cell anergy
negative regulation of immune system process
positive regulation of immune system process
cytoplasmic pattern recognition receptor signaling pathway
T cell anergy
ubiquitin-dependent protein catabolic process
protein monoubiquitination
apoptotic process
inflammatory response
response to oxidative stress
positive regulation of catabolic process
regulation of gene expression
positive regulation of macromolecule metabolic process
protein ubiquitination
protein catabolic process
receptor internalization
negative regulation of type I interferon production
protein K29-linked ubiquitination
CD4-positive, alpha-beta T cell proliferation
nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway
CXCL12-activated CXCR4 signaling pathway
negative regulation of viral-induced cytoplasmic pattern recognition receptor signaling pathway
negative regulation of apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
innate immune response
positive regulation of protein catabolic process
negative regulation of JNK cascade
viral entry into host cell
negative regulation of defense response to virus
negative regulation of multicellular organismal process
defense response to virus
protein autoubiquitination
regulation of necroptotic process
protein K63-linked ubiquitination
protein K48-linked ubiquitination
regulation of protein deubiquitination
protein branched polyubiquitination
regulation of hematopoietic stem cell differentiation
negative regulation of intracellular signal transduction
negative regulation of CD4-positive, alpha-beta T cell proliferation
positive regulation of receptor catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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