Invitrogen

ITGA10 Polyclonal Antibody

1个已发表图片

1 篇参考文献

查看其他4个ITGA10抗体

问题与答案

问题与答案

问题与答案

已发表图片 (1)

ITGA10 Antibody in Western Blot (WB)

ITGA10 Antibody in Western Blot (WB)

图: 1 / 1

ITGA10 Antibody (PA5-67829) in WB

International journal of molecular medicine 2021 - Figure 4 Kaiso regulates the PI3K/AKT pathway via Itga10. Western blot analysis of Itga10 expression in (A) Kaiso-overexpressing or (B) Kaiso-knockdown MC3T3-E1 cells. (C) Chromatin immunoprecipitation analysis of the binding of Kaiso to the promoter of Itga10 in LV-ctr and LV-Kaiso MC3T3-E1 cells. Input DNA and DNA immunoprecipitated with IgG were included as positive and negative controls, respectively. T... View More

ITGA10 Antibody in Western Blot (WB)

Product Image Gallery

ITGA10 Antibody in Western Blot (WB) — Figure 4 Kaiso regulates the PI3K/AKT pathway via Itga10. Western blot analysis of Itga10 expression in (A) Kaiso-overexpressing or (B) Kaiso-knockdown MC3T3-E1 cells. (C) Chromatin immunoprecipitation analysis of the binding of Kaiso to the promoter of Itga10 in LV-ctr and LV-Kaiso MC3T3-E1 cells. Input DNA and DNA immunoprecipitated with IgG were included as positive and negative controls, respectively. The results are expressed as percentages of the input level. (D) WT and (E) Kaiso binding site-MUT Itga10 promoter-luciferase reporter vectors were co-transfected with Kaiso-overexpression vectors or control vector in MC3T3-E1 cells. Luciferase activity was normalized to Renilla luciferase activity and presented as the fold change to LV-ctr. (F) Expression levels of p85, AKT and p-AKT were determined via western blot analysis in Sh-Itga10-1, Sh-Itga10-2 and Sh-ctr transfectedMC3T3-E1 cells. (G) RT-qPCR analysis of ALP, OCN and BSP expression levels in Sh-ctr and Sh-Kaiso transfected MC3T3-E1 cells after 7 days culture with osteogenic medium. (H) ALP activity was measured on day 7. (I) ALP staining was performed on day 7, and Alizarin Red S staining was performed on day 21. All quantitative data are presented as the mean +- SD (n=3). ** P<0.01. WT, wild-type; MUT, mutant; LV, lentivirus; shRNA, short hairpin RNA; Ctr, control; p-, phosphorylated; ALP, alkaline phosphatase; OCN, osteocalcin; BSP, bone sialoprotein; Itga10, integrin subunit alpha10; RT-qPCR, reverse transcripti

Immunogen image

ITGA10 Polyclonal Antibody

Immunogen Image ITGA10 Polyclonal Antibody

产品信息

PA5-67829

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthesized peptide derived from human ITGA10.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Predicted to react with Mouse samples.

靶标信息

Integrin alpha-10/beta-1 is a receptor for collagen.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: integrin alpha 10; Integrin alpha-10; integrin, alpha 10

查看更多收起

基因别名: A630048L14; ITGA10; PRO827; UNQ468/PRO827

查看更多收起

UniProt ID: (Human) O75578

查看更多收起

Entrez Gene ID: (Human) 8515, (Mouse) 213119

查看更多收起

collagen binding metal ion binding collagen binding involved in cell-matrix adhesion integrin

cell-matrix adhesion integrin-mediated signaling pathway extracellular matrix organization