Invitrogen

ITGB5 Recombinant Rabbit Monoclonal Antibody (PSH10-13)

Name: ITGB5 Recombinant Rabbit Monoclonal Antibody (PSH10-13)
Brand: Invitrogen
SKU: MA562916

高级验证

查看其他20个ITGB5 (Integrin beta 5)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (7)
高级验证 (3)

ITGB5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ITGB5 Antibody (MA562916) in IHC (P)

Immunohistochemical analysis of ITGB5 was performed using formalin-fixed paraffin-embedded human colon adenocarcinoma tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 1... View More

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ITGB5 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell line models owing to their inherent genetic constitution. Western blot using ITGB5 Recombinant Rabbit Monoclonal Antibody (PSH10-13) (Product # MA562916) shows high expression of ITGB5 in PANC-1 which expresses higher levels of ITGB5, and no expression in Ramos which expresses lower levels of ITGB5. {RE}

Immunogen image

Immunogen Image ITGB5 Recombinant Rabbit Monoclonal Antibody (PSH10-13)

产品信息

MA562916

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

产品规格

种属反应

Human, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH10-13

抗原

Recombinant protein within human Integrin beta 5 aa 1-719.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control: A549 cell lysate, HT-29 cell lysate, NCI-H1299 cell lysate, PANC-1 cell lysate, COS-1 cell lysate, C6 cell lysate, Rat lung tissue lysate, Rat kidney tissue lysate.

Tissue Specificity: Low tissue specificity.

Subcellular Location: Cell membrane.

Sequence Similarities: 91% Mouse/Rat.

Predicted band size: 88 kDa

靶标信息

Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around 22 different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in-groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。