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Invitrogen
ITPKA Polyclonal Antibody
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FIGURE: 1 / 2
ITPKA Antibody (PA5-101093) in ICC/IF
Immunocytochemistry analysis of ITPKA in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ITPKA polyclonal antibody (Product # PA5-101093) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-101093
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ITPKA(Accession P23677), corresponding to amino acid residues L37-E87.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total IP3KA.
Target Information
Regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 2.7.1.127; inositol 1,4,5-triphosphate 3-kinase; inositol 1,4,5-trisphosphate 3-kinase A; inositol 145-triphosphate 3-kinase; insP 3-kinase A; IP3 3 kinase A; IP3 3-kinase A; IP3-kinase A; IP3K A
Gene Aliases: IP3-3KA; IP3KA
UniProt ID: (Human) P23677, (Mouse) Q8R071, (Rat) P17105
Entrez Gene ID: (Human) 3706, (Mouse) 228550, (Rat) 81677
signal transduction
inositol phosphate biosynthetic process
inositol phosphate metabolic process
phosphatidylinositol phosphorylation
response to calcium ion
cellular response to calcium ion
modification of postsynaptic actin cytoskeleton
inositol metabolic process
actin cytoskeleton organization
regulation of synaptic plasticity
positive regulation of dendritic spine morphogenesis
dendritic spine maintenance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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