Invitrogen

IkB alpha Recombinant Rabbit Monoclonal Antibody (1I1F7)

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说明书
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说明书
实验方案
问题与答案
技术支持
IkB alpha Recombinant Rabbit Monoclonal Antibody (1I1F7)

产品信息

MA5-58142

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000-1:2,000
-

酶联免疫吸附实验 (ELISA)

1 µg/mL
-
产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1I1F7

抗原

Recombinant protein (or fragment)
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.73 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Dry ice (international)

RRID

AB_3681164

产品详细信息

Immunogen sequence: MFQPAGHGQD WAMEGPRDGL KKERLVDDRH DSGLDSMKDE DYEQMVKELR EIRLQPQEAP LAAEPWKQQL TEDGDSFLHL AIIHEEKTLT MEVIGQVKGD LAFLNFQNNL QQTPLHLAVI TNQPGIAEAL LKAGCDPELR DFRGNTPLHL ACEQGCLASV AVLTQTCTPQ HLHSVLQATN YNGHTCLHLA SIHGYLGIVE HLVTLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQTL PESEDEESYD TESEFTEDEL PYDDCVFGGQ RLTL

靶标信息

IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: I(Kappa)B(alpha); I-kappa-B-alpha; I-kappaBalpha; IkappaBalpha; IkB-alpha; IKBalpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; RL/IF-1

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基因别名: AI462015; IKBA; MAD-3; MAD3; NFKBI; NFKBIA; RL/IF-1

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UniProt ID: (Human) P25963, (Rat) Q63746, (Mouse) Q9Z1E3

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Entrez Gene ID: (Human) 4792, (Rat) 25493, (Mouse) 18035

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功能
protein binding transcription factor binding nuclear localization sequence binding enzyme binding heat shock protein binding ubiquitin protein ligase binding protein complex binding identical protein binding NF-kappaB binding
参与通路
protein import into nucleus, translocation cytoplasmic sequestering of NF-kappaB regulation of gene expression negative regulation of macrophage derived foam cell differentiation positive regulation of cholesterol efflux negative regulation of lipid storage lipopolysaccharide-mediated signaling pathway negative regulation of NF-kappaB transcription factor activity positive regulation of cellular protein metabolic process response to muramyl dipeptide response to lipopolysaccharide toll-like receptor 4 signaling pathway response to muscle stretch regulation of cell proliferation cytoplasmic sequestering of transcription factor response to exogenous dsRNA negative regulation of myeloid cell differentiation negative regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter nucleotide-binding oligomerization domain containing 1 signaling pathway nucleotide-binding oligomerization domain containing 2 signaling pathway stimulatory C-type lectin receptor signaling pathway apoptotic process viral process Fc-epsilon receptor signaling pathway regulation of NF-kappaB import into nucleus negative regulation of apoptotic process negative regulation of DNA binding T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity cellular response to cold cellular response to cytokine stimulus cellular response to organic cyclic compound
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