Invitrogen

Insulin Polyclonal Antibody

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问题与答案

问题与答案

抗体检测数据 (6)

Insulin Antibody in Immunocytochemistry (ICC/IF)

Insulin Antibody in Immunocytochemistry (ICC/IF)

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Insulin Antibody (PA5149828) in ICC/IF

Immunocytochemistry analysis of Insulin in Hepa1-6 cells. Samples were incubated with Insulin Polyclonal (Product # PA5149828) antibody using a dilution of 20 µg/mL, followed by AF488-Linked Caprine Anti-Rabbit IgG (2 µg/mL).

Insulin Antibody in Immunocytochemistry (ICC/IF)

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Insulin Antibody in Western Blot (WB)

Insulin Antibody in Western Blot (WB)

Product Image Gallery

Insulin Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Insulin in Hepa1-6 cells. Samples were incubated with Insulin Polyclonal (Product # PA5149828) antibody using a dilution of 20 µg/mL, followed by AF488-Linked Caprine Anti-Rabbit IgG (2 µg/mL).

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Insulin in paraffin-embedded Mouse Pancreas tissue; (Control: PBS). Samples were incubated with Insulin Polyclonal (Product # PA5149828) antibody using a dilution of 10 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG (2 µg/mL) and DAB.

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Insulin in paraffin-embedded Rat Pancreas tissue; (Control: PBS). Samples were incubated with Insulin Polyclonal (Product # PA5149828) antibody using a dilution of 10 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG (2 µg/mL) and DAB.

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Insulin in paraffin-embedded Mouse Stomach tissue. Samples were incubated with Insulin Polyclonal (Product # PA5149828) antibody, followed by DAB.

Insulin Antibody in Western Blot (WB) — Western blot analysis of Insulin in Lane 1: Mouse Pancreas tissue; Lane 2: Mouse Testis tissue; Lane 3: Mouse Liver tissue. Samples were incubated with Insulin Polyclonal (Product # PA5149828) antibody.

Insulin Antibody in Western Blot (WB) — Western blot analysis of Insulin in recombinant protein. Samples were incubated with Insulin Polyclonal (Product # PA5149828) antibody.

Immunogen image

Insulin Polyclonal Antibody

Immunogen Image Insulin Polyclonal Antibody

产品信息

PA5149828

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.01-2 µg/mL

-

免疫组化（石蜡） (IHC (P))

5-20 µg/mL

-

免疫细胞化学 (ICC/IF)

5-20 µg/mL

-

产品规格

种属反应

Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

Recombinant Insulin (INS) (Phe25-Ser54 and Gly90-Asn110 linked by GGGGS)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Ins; Insulin-1; Insulin-2

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基因别名: AA986540; Ins-1; Ins-2; Ins1; Ins2; Ins2-rs1; InsII; Mody; Mody4; proinsulin

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UniProt ID: (Mouse) P01325, (Mouse) P01326

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Entrez Gene ID: (Mouse) 16333, (Mouse) 16334

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protease binding insulin receptor binding hormone activity chaperone binding insulin-like growth factor receptor binding protein complex binding identical protein binding growth factor

carbohydrate metabolic process glucose metabolic process glucose transport negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity negative regulation of acute inflammatory response regulation of cellular amino acid metabolic process acute-phase response ER overload response G-protein coupled receptor signaling pathway activation of JUN kinase activity myoblast fusion positive regulation of cell proliferation insulin receptor signaling pathway regulation of gene expression positive regulation of phosphatidylinositol 3-kinase signaling myotube differentiation lipid catabolic process regulation of transmembrane transporter activity activation of protein kinase B activity positive regulation of cellular protein metabolic process negative regulation of protein oligomerization regulation of protein localization negative regulation of NAD(P)H oxidase activity wound healing negative regulation of protein catabolic process regulation of phosphorylation glucose homeostasis regulation of protein binding positive regulation of MAPK cascade positive regulation of phosphatidylinositol 3-kinase activity positive regulation of glycogen biosynthetic process positive regulation of DNA replication negative regulation of glycogen catabolic process positive regulation of glycolytic process positive regulation of mitotic nuclear division negative regulation of proteolysis negative regulation of fatty acid metabolic process positive regulation of glucose import positive regulation of insulin receptor signaling pathway alpha-beta T cell activation regulation of protein secretion negative regulation of protein secretion positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipid catabolic process positive regulation of NF-kappaB transcription factor activity positive regulation of protein kinase B signaling fatty acid homeostasis canonical Wnt signaling pathway negative regulation of respiratory burst involved in inflammatory response positive regulation of respiratory burst intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress positive regulation of protein localization to nucleus negative regulation of feeding behavior