Invitrogen

Integrin beta 5 Recombinant Rabbit Monoclonal Antibody (8D2B1)

Name: Integrin beta 5 Recombinant Rabbit Monoclonal Antibody (8D2B1)
Brand: Invitrogen
SKU: MA5-55281

查看其他20个ITGB5 (Integrin beta 5)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Integrin beta 5 Antibody in Western Blot (WB)

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Integrin beta 5 Antibody (MA5-55281) in WB

Western blot analysis of lysates from A549 cells, using Integrin beta 5 Recombinant Monoclonal Antibody (Product # MA5-55281) at 1:1000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Product Image Gallery

Immunogen image

Immunogen Image Integrin beta 5 Recombinant Rabbit Monoclonal Antibody (8D2B1)

产品信息

MA5-55281

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

8D2B1

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 24-490 of human Integrin beta5/ITGB5.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.36 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.05% ProClin 300

保存条件

-20°C, Avoid Freeze/Thaw Cycles

AB_3667523

产品详细信息

Immunogen Sequence: GLNICTSGSA TSCEECLLIH PKCAWCSKED FGSPRSITSR CDLRANLVKN GCGGEIESPA SSFHVLRSLP LSSKGSGSAG WDVIQMTPQE IAVNLRPGDK TTFQLQVRQV EDYPVDLYYL MDLSLSMKDD LDNIRSLGTK LAEEMRKLTS NFRLGFGSFV DKDISPFSYT APRYQTNPCI GYKLFPNCVP SFGFRHLLPL TDRVDSFNEE VRKQRVSRNR DAPEGGFDAV LQAAVCKEKI GWRKDALHLL VFTTDDVPHI ALDGKLGGLV QPHDGQCHLN EANEYTASNQ MDYPSLALLG EKLAENNINL IFAVTKNHYM LYKNFTALIP GTTVEILDGD SKNIIQLIIN AYNSIRSKVE LSVWDQPEDL NLFFTATCQD GVSYPGQRKC EGLKIGDTAS FEVSLEARSC PSRHTEHVFA LRPVGFRDSL EVGVTYNCTC GCSVGLEPNS ARCNGSGTYV CGLCECS

靶标信息

Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around 22 different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in-groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。