Invitrogen

Integrin beta 5 Monoclonal Antibody (KN52), FITC, eBioscience™

Name: Integrin beta 5 Monoclonal Antibody (KN52), FITC, eBioscience\u2122
Brand: Invitrogen
SKU: 11-0497-42

3个已发表图片

9 篇参考文献

查看其他20个ITGB5 (Integrin beta 5)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (3)

Integrin beta 5 Antibody in Flow Cytometry (Flow)

图: 1 / 4

Integrin beta 5 Antibody (11-0497-42) in Flow

Staining of the Chinese Hamster Ovary (CHO) cell line with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Integrin beta 5 FITC (filled histogram). Total viable cells were used for analysis.

Integrin beta 5 Antibody in Immunocytochemistry (ICC/IF)

Integrin beta 5 Antibody in Neutralization (Neu)

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产品信息

11-0497-42

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看5篇已发表文章 5篇已发表文章

中和实验 (Neu)

查看1篇已发表文章 1篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Hamster, Human, Mouse

已发表种属

C. elegans, Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

KN52

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10547284

产品详细信息

Description: The KN52 antibody reacts with human integrin beta 5, an approximately 100,000 Mr (reduced) and 95,000 Mr (nonreduced) a member of the integrin family. Only a single alpha chain, the alpha v subunit, associates with the integrin beta 5 to form the vitronectin receptor complex. Integrin alpha v/beta 5 complex also binds to the basic domain of Tat (he sequence RKKRRQRRR). The integrin beta 5 is found on carcinoma cell lines, hepatoma and fibroblast cell lines, and is absent from lymphoblastoid cells and platelets.

The KN52 monoclonal antibody has been shown to inhibit migration.

Applications Reported: This KN52 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This KN52 antibody has been pre-titrated and tested by flow cytometric analysis of Chinese Hamster Ovary (CHO), A549 and normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around 22 different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in-groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。