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Agrisera
JAR1/Jasmonic acid-amido synthetase JAR1 Polyclonal Antibody
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JAR1/Jasmonic acid-amido synthetase JAR1 Antibody (AS16-3688) in WB
5 µg of protein from Arabidopsis thaliana Col 0 and jar 1-1 mutant were extracted using basic phenol protocol. 1 g were grinded with liquid nitrogen with mortar and pestle, powder were resuspended in 3 m L of protein buffer (0.5 M Tris-HCl, 0.7 M sucrose, 1 m M PMSF, 50 m M EDTA, 0.1 M KCl and 0.2% β-mercaptoethanol; p H 8.0). Homogenate were centrifuged at 8,000 rpm and supernatant were mixed with three vo... View More
产品信息
AS16-3688
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana JAR1 protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
JAR1 encodes a soluble, cytosolic GH3-family acyl acid-amido synthetase of the firefly luciferase-like adenylate-forming enzyme superfamily that functions in jasmonate hormone bioactivation in plants. The gene produces a protein of roughly mid-60 kDa size that contains the conserved GH3/adenylate-forming catalytic architecture used to activate carboxylate substrates with ATP (via an acyl-adenylate intermediate) prior to amide bond formation with amino acids. Biochemically, JAR1 preferentially conjugates jasmonic acid to L-isoleucine to generate jasmonoyl-L-isoleucine (JA-Ile), a principal bioactive jasmonate that drives downstream signaling by promoting COI1-dependent perception and subsequent turnover of JAZ transcriptional repressors, thereby enabling rapid transcriptional reprogramming during wounding and defense responses. Genetic disruption of JAR1 reduces JA-Ile accumulation and impairs multiple jasmonate-dependent phenotypes, consistent with a central role in converting the jasmonate precursor pool into an active signal that coordinates stress adaptation and aspects of development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AtGH3.11; F11C10.6; FAR-RED INSENSITIVE 219; FIN219; JASMONATE RESISTANT 1
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