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Agrisera
JAZ1/Jasmonate ZIM-domain protein 1 Polyclonal Antibody
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JAZ1/Jasmonate ZIM-domain protein 1 Antibody (AS13-2648) in WB
JAZ 1 HA was transiently expressed in Arabidopsis thaliana protoplast for 6 hours. 200 µL of protoplast cells were harvested and mixed with 100 µL 2 X SDS buffer. Proteins were separated on 4-12% Nu PAGE gel and blotted for 1 hr to PVDF using the Invitrogen XCell II blot module. Blot was blocked with 5% non-fat milk in TBST buffer for 1 hr at room temperature with agitation. Blot was incubated in the primar... View More
产品信息
AS13-2648
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana JAZ1 protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water in 40% glycerol to a final protein concentration of 100 ng/µL.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
JAZ1, also called Jasmonate ZIM domain protein 1, is a transcriptional repressor in the jasmonic acid signaling pathway in plants. In the absence of jasmonoyl isoleucine, JAZ1 binds transcription factors such as MYC2 to prevent activation of defense and growth response genes. When jasmonoyl isoleucine accumulates, it promotes COI1 mediated recognition of JAZ1, leading to ubiquitination and proteasomal degradation, which releases transcription factors to activate jasmonate responsive programs. JAZ1 contains a ZIM domain for dimerization and interactions with co repressors like NINJA and TPL and a Jas motif required for COI1 binding. Through these interactions, JAZ1 integrates stress cues, hormone cross talk, and developmental signals to fine tune defense, secondary metabolism, and growth tradeoffs in different tissues and conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AtJAZ1; jasmonate-zim-domain protein 1; T29M8.5; T29M8_5; TIFY10A
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