Invitrogen

JPH1 Polyclonal Antibody

Name: JPH1 Polyclonal Antibody
Brand: Invitrogen
SKU: 40-5100

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18个已发表图片

22 篇参考文献

查看其他7个JPH1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

JPH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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JPH1 Antibody (40-5100) in IHC (P)

Immunohistochemistry analysis of Junctophilin-1 showing staining in the cytoplasm of paraffin-embedded mouse skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at ro... View More

JPH1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

JPH1 Antibody — Figure 3 Proteolysis of JP1 in intensively stimulated muscles A , Western blot of JP1 in fibres from rat EDL muscles stimulated intensively in vitro in the presence of 30 m m caffeine (Stim) and from non-stimulated contralateral muscles (Con); three fibre segments were pooled in each lane. Proteins were separated on 4-15% Stain Free gels and probed for JP1 using mid-region or C-terminal antibody, and also antibody to RyR1. B , mean (+SEM) amount of 75 kDa JP1 fragment in fibre samples from Stim and Con muscles, expressed as a percentage of the total JP1 detected (i.e. the sum of the 75 and 90 kDa bands). The number of samples is shown with each bar, together with the number of muscles in parentheses (** P < 0.01, Mann-Whitney U test). C , mean (+SEM) amount of 15 kDa JP1 fragment in samples (each value was first normalized to MHC). D , mean (+SEM) amount of RyR1 in samples. E , Western blot of JP1 and the 75 and 15 kDa proteolytic fragments remaining in skinned fibre (F-sk) or diffusing into wash solution (Diff) after bathing skinned fibre segments in wash solution for 5 min (see Methods). Fibres were obtained from stimulated muscle; three skinned segments were used in each of two wash-fibre sets shown. A substantial proportion of the 75 kDa proteolytic fragments diffused into the wash solution (arrows). {TM}

Immunogen image

Immunogen Image JPH1 Polyclonal Antibody

产品信息

40-5100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-3 µg/mL

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免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50

查看1篇已发表文章 1篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫组化（冰冻） (IHC (F))

Assay-dependent

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免疫细胞化学 (ICC/IF)

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其他PubMed (Misc)

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产品规格

种属反应

Mouse, Rat

已发表种属

C. elegans, Human, Mouse, Rabbit, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the C-terminal region of the mouse junctophilin-1 (JP-1, junctophilin type 1, JPH1) protein, which differs from rat by one non-conservative amino acid replacement

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533470

靶标信息

Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family.

仅用于科研。不用于诊断过程。未经明确授权不得转售。