Invitrogen

Jagged1 Monoclonal Antibody (J.841.5)

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Jagged1 Antibody in Western Blot (WB)

Jagged1 Antibody in Western Blot (WB)

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Jagged1 Antibody (MA5-15012) in WB

Western blot analysis of Jagged1 in total cell lysates from HepG2 and LNCaP cells using Jagged1 monoclonal antibody (Product # MA5-15012).

Jagged1 Antibody in Western Blot (WB)

Jagged1 Antibody in Western Blot (WB)

Product Image Gallery

Jagged1 Antibody in Western Blot (WB) — Western blot analysis of Jagged1 in total cell lysates from HepG2 and LNCaP cells using Jagged1 monoclonal antibody (Product # MA5-15012).

Jagged1 Antibody in Western Blot (WB) — Figure 3 Classical macrophage activation is associated with elevated Notch ligand Jagged1 expression in macrophages and activation of Notch signalling in cocultured HPCs. (a) Quantitative analysis of Notch ligands ( Jag1 , Jag2 , Dll1 , and Dll4 ) expression in M0, M1, and M2 macrophages. (b) Jagged1 levels on M1 macrophages are confirmed by flow cytometry and Western blots. Flow cytometry results are represented by scatter plot, and calculated flow cytometry data are represented; * p < 0.05 vs M0 macrophages and # p < 0.05 vs M2 macrophages. (c) Histograms from flow cytometry analysis represent Jagged1 expressions in M1 macrophages from EGFP transgenic C57BL/6 mice cultured with or without HPC for 24 hours. (d) Graphs show relative mRNA expression levels of HES1 in HPC alone or in HPC cocultured with M0, M1, or M2 macrophages; levels of HES1 staining (Alexa Flour 568) in HPC were determined by static cytometry. Graphs show calculated relative expression of HES1 in HPC cocultured with M0, M1, or M2 macrophages (percentage of HPC culture alone). * p < 0.05 vs HPC alone, # p < 0.05 vs M0 macrophages cocultured with HPC, and SS p < 0.05 vs M2 macrophages cocultured with HPC. (c) Representative Western blots showing HES1 protein levels in HPC after coculturing with M0, M1, or M2 macrophages.

产品信息

MA5-15012

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫沉淀 (IP)

1:50

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产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

J.841.5

抗原

Synthetic peptide corresponding to residues surrounding Glu1140 (intracellular region) of human Jagged1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

78 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10986733

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with Jagged2.

靶标信息

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

M1-Polarized Macrophages Promote Self-Renewing Phenotype of Hepatic Progenitor Cells with Jagged1-Notch Signalling Involved: Relevance in Primary Sclerosing Cholangitis.

Journal of immunology research

Li H,Sun S,Lei Q,Lei P,Cai X,Wan C,Shen G

Published figure using Jagged1 monoclonal antibody (Product # MA5-15012) in Western Blot

Mon Apr 08 00:00:00 EDT 2019

生物信息学

蛋白别名: CD339; jagged canonical Notch ligand 1; Jagged1; Protein jagged-1; Serrate-1

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基因别名: ABE2; AGS; AHD; AWS; CD339; Gsfabe2; HJ1; Htu; JAG1; JAGL1; Ozz; Ser-1

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UniProt ID: (Human) P78504, (Mouse) Q9QXX0

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Entrez Gene ID: (Human) 182, (Mouse) 16449

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Notch binding structural molecule activity calcium ion binding protein binding growth factor activity

参与通路

angiogenesis cell fate determination blood vessel remodeling morphogenesis of an epithelial sheet T cell mediated immunity pulmonary valve morphogenesis cardiac right ventricle morphogenesis Notch signaling pathway Notch receptor processing nervous system development hemopoiesis keratinocyte differentiation regulation of cell migration response to muramyl dipeptide aorta morphogenesis regulation of cell proliferation auditory receptor cell differentiation myoblast differentiation endothelial cell differentiation negative regulation of fat cell differentiation positive regulation of myeloid cell differentiation negative regulation of neuron differentiation positive regulation of osteoblast differentiation positive regulation of Notch signaling pathway positive regulation of transcription from RNA polymerase II promoter cardiac septum morphogenesis ciliary body morphogenesis pulmonary artery morphogenesis cardiac neural crest cell development involved in outflow tract morphogenesis Notch signaling involved in heart development endocardial cushion cell development nephron development glomerular visceral epithelial cell development distal tubule development loop of Henle development neuronal stem cell population maintenance negative regulation of stem cell differentiation cell communication multicellular organism development organ morphogenesis camera-type eye development negative regulation of cell differentiation inner ear development