Invitrogen

KAP3 Polyclonal Antibody

Name: KAP3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-54878

高级验证

查看其他11个KAP3抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (1)

KAP3 Antibody in Immunocytochemistry (ICC/IF)

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KAP3 Antibody (PA5-54878) in ICC/IF

Immunofluorescence analysis of KAP3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KAP3 Polyclonal Antibody (Product # PA5-54878) at 5µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a di... View More

KAP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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KAP3 Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. SH-SY5Y cells were transfected with KAP3 siRNA and loss of signal was observed in Western Blot using KAP3 Polyclonal Antibody (Product # PA5-54878). {KD}

Immunogen image

Immunogen Image KAP3 Polyclonal Antibody

产品信息

PA5-54878

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human KAP3. Recombinant protein control fragment (Product #RP-94021).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.05 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2642876

产品详细信息

Immunogen sequence: ELLNAQQEDD EFVCQIIYVF YQMVFHQATR DVIIKETQAP AYLIDLMHDK NNEIRKVCDN TLDIIAEYDE EWAKK

Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

靶标信息

The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。