Invitrogen

KCC2 Polyclonal Antibody

KCC2 Antibody in Immunocytochemistry (ICC/IF)

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KCC2 Antibody (PA5-78544) in ICC/IF

KCC2 Polyclonal Antibody detects KCC2 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: KCC2 protein stained by KCC2 Polyclonal Antibody (Product # PA5-78544) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT1338]diluted at 1:500. Blue: Fluoroshield with DAPI .

KCC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-78544

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human KCC2. The exact sequence is proprietary.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.36 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2735976

产品详细信息

Positive Control: SK-N-SH, mouse hippocampus, rat hippocampus, rat spinal cord

Predicted Reactivity: Mouse (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Impaired KCC2 Function Triggers Interictal-Like Activity Driven by Parvalbumin-Expressing Interneurons in the Isolated Subiculum In Vitro.

Cerebral cortex (New York, N.Y. : 1991)

Anstötz M,Fiske MP,Maccaferri G

PA5-78544 was used in Immunohistochemistry to show that reduced KCC2 activity in subicular networks in vitro is sufficient to induce interictal-like activity via altered GABAergic signaling from PVs without other epilepsy-related changes.

Thu Aug 26 00:00:00 EDT 2021

生物信息学

蛋白别名: Electroneutral potassium-chloride cotransporter 2; erythroid K-Cl cotransporter 2; Furosemide-sensitive K-Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2a variant 1; KCC2a-S25 variant 1; KCC2b variant 2; mKCC2; Neuronal K-Cl cotransporter; neuronal-specific K-Cl cotransporter; rKCC2; S12A5; solute carrier family 12 (potassium/chloride transporter), member 5; Solute carrier family 12 member 5; solute carrier family 12, member 5

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基因别名: EIEE34; EIG14; hKCC2; KCC2; KIAA1176; mKIAA1176; SLC12A5

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UniProt ID: (Human) Q9H2X9, (Mouse) Q91V14, (Rat) Q63633

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Entrez Gene ID: (Human) 57468, (Mouse) 57138, (Rat) 171373

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功能

chloride transmembrane transporter activity potassium:chloride symporter activity potassium ion symporter activity protein binding ammonium transmembrane transporter activity symporter activity cation:chloride symporter activity protein kinase binding secondary carrier transporter

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